抗体

Cyclin D1(细胞周期素D1) 兔多克隆抗体

基本信息
产品名称:
Cyclin D1(细胞周期素D1) 兔多克隆抗体
英文名称:
Cyclin D1
国产/进口:
国产
产地/品牌:
华安生物/HUABIO
型号:
兔多克隆抗体
参考报价:
2500/100ul
总点击数:
3088
更新日期:
2019-12-09
产品类别:

性能参数
其他名称 Cyl-1、Ccnd1、G1/S-specific cyclin-D1
 
抗体类型 兔多抗 产品类型 一抗
反应种属 Zebrafish , Rat , Mouse , Human 产品应用 WB
储藏条件   产品性状 液体
分子量 36kDa 克隆号  
保质期 12个月 产品品牌 华安
细胞定位 细胞质 阳性对照 MCF-7

抗原介绍
细胞周期素D1(Cyclin D1)是一种与细胞G1期相关的蛋白质,作用于G1期的细胞,含有295个氨基酸,由染色体11q13上的CCND1基因编码,分子量为36kDa,与多种蛋白相互作用促进细胞进入S期。Cyclin D1基因是一种弱致癌基因,可与多种癌基因协同促进细胞转化。在多种肿瘤中有Cyclin D1基因扩增和/或表达增强,其临床意义与肿瘤类型有关。用Cyclin D1反义基因体外转染人类肿瘤细胞可抑制肿瘤细胞增殖,降低其致瘤性。

应用介绍
【免疫原】免疫原为KLH-多肽交联物,多肽位于C-末端附近区域
【推荐使用浓度】WB: 1:10,000

Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

 
 

Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

 
 

Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

 
 

Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

公司简介

杭州华安生物技术有限公司成立于2007年3月,总部位于中国杭州,是一家从事抗体研发与生产的国家高新技术企业。公司致力于为全球的科研工作者和工业客户提供高品质的抗体试剂及抗体服务。
    公司的产品线包括兔单抗、小鼠单抗、兔多抗、二抗、蛋白/多肽、基因敲除细胞、抗体检测用相关试剂等。2015年,公司开始专注于高通量重组兔单克隆抗体的研发与产业化生产。
    同时,公司也为客户提供定制抗体开发、纯化和筛选服务迄今为止,公司累计完成超过 3000个定制抗体项目,涉及植物、动物、医学、水产、病毒等不同的科研领域。用户使用公司抗体在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等杂志上发表文章。
    公司不但专注于自身技术体系的完善和升级,还注重推动产学研合作:与浙江大学、四川大学、西南大学、中国科学院遗传与发育生物学研究所等知名院校共同承担省级和国家级课题,与杭州精准医药研究中心进行深度合作,进行技术成果交流和共享。

 

Hangzhou HuaAn Biotechnology Co., Ltd (HuaBio) was founded in 2007. Our headquarter is located in Hangzhou, China. With over 3,000 antibodies developed, HuaBio is committed to providing high-quality antibodies and antibody services to our customers. We have products including recombinant rabbit monoclonal antibodies, mouse monoclonal antibodies, rabbit polyclonal antibodies, phosphor-specific antibodies, secondary antibodies.

HuaBio is also committed to providing the highest quality in customized antibody services . By now, we have delivered more than 3000 customized antibodies for specific applications in various fields (e.g.Neuroscience, Epigenetics, Stem Cells, Microbiology, Signal Transduction Apoptosis and etc. )


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