N6培养基被定义用来改善水稻花粉愈伤组织的形成、生长和分化。培养基中铵盐的浓度对于愈伤组织的形成是至关重要的。NH4+离子浓度的最适范围是7.0 mM（或3.5mM(NH4)2SO4)）。高浓度的铵盐会显著抑制水稻花粉的生长和分化。KNO3和培养基中其他成分的浓度不会影响花粉的生长。
Chu C.C, The N6 medium and its application to anther culture of cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).
Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic., 18, 659 (1975).