多因子、高通量筛选 之Antibody Array抗体芯片
2015-12-28 来源:本站 点击次数:1167样本珍贵，不易得，想多检测几个因子，量不够，怎么办？
想检测的因子太多，不想一次次的重复试验，怎么办？
经费太少，还没怎么用，就快没了，怎么办？
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多因子、高通量筛选 之Antibody Array芯片平台
优宁维代理的R&Dsystems的抗体芯片是ELISA原理的高通量WB，每个芯片在设计时都利用了精心挑选的捕获抗体，它们在硝酸纤维素膜上重复点样两次。 多因子、高通量筛选 之Antibody Array芯片平台
▍抗体芯片平台介绍：
通过监控蛋白水平和一些蛋白翻译后修饰的变化，能迅速靶定目标分子，以便进一步的研究。R&DSystems的蛋白芯片能广泛地应用于信号转导，血管再生，细胞凋亡，肿瘤，肥胖，以及干细胞，药物筛选等的研究。
蛋白芯片 能同时检测单个样品中多种分析物的相对水平。蛋白芯片的使用不需要任何特殊的仪器，也取代了多次免疫沉淀/ Western blot实验，蛋白芯片高通量定性分析试剂盒比WB的灵敏度高100倍；更有大规模多分子高通量筛选作用，经多项研究证实，蛋白芯片能简化鉴定蛋白所需的筛选过程。
▍抗体芯片特点：
◆ 多样性： 一张膜可检测15-119个目标蛋白；
◆ 高效率：所需样品量少，大大缩短操作时 间，有效节约成本；
◆ 操作简单：即用型产品，无需购买抗体，无需特殊仪器
◆ 高品质：高特异性和低批间差距保证样品的检测结果的 稳定性；
▍抗体芯片实验流程：
▍抗体芯片和WB比较如下：
| WB | ARRAY | |
| 结果 | 定性 | 定性 |
| 通量 | 10-20 data points/membrane | 15-119 data points/membrane(根据膜种类而定） |
| 灵敏度 | 低 | 高 |
| 操作时间 | 5-6h + Overnight | 2.5-3h+ Overnight |
| 工作量： 8 samples*20 因子 |
20 membranes | 8 membranes |
▍部分芯片产品清单，若需要详细清单请 联系我们：
| ARY001B: human phospho-RTK | ||||
| 1×4 membranes | ||||
| Sample Type:cell lysate | ||||
| ALK/CD246 | EphA5 | ErbB3 | M-CSF R | SCF R/c-kit |
| Axl | EphA6 | ErbB4 | Mer | Tie-1 |
| DDR1 | EphA7 | FGF R1 | MSP R/Ron | Tie-2 |
| DDR2 | EphA10 | FGF R2 alpha | MuSK | TrkA |
| Dtk | EphB1 | FGF R3 | PDGF R alpha | TrkB |
| EGF R | EphB2 | FGF R4 | PDGF R beta | TrkC |
| EphA1 | EphB3 | Flt-3/Flk-2 | c-Ret | VEGF R1/Flt-1 |
| EphA2 | EphB4 | HGF R/c-MET | ROR1 | VEGF R2/KDR |
| EphA3 | EphB6 | IGF-I R | ROR2 | VEGF R3/Flt-4 |
| EphA4 | ErbB2 | Insulin R/CD220 | Ryk | |
| ARY002B: human phospho-MAPK | ||||
| 1×4 membranes | ||||
| Sample Type: cell lysate | ||||
| Akt1 | ERK2 | JNK3 | p38 beta | RSK2 |
| Akt2 | GSK-3 alpha/beta | JNK pan | p38 delta | TOR |
| Akt3 | GSK-3 beta | MKK3 | p38 gamma | |
| Akt pan | HSP27 | MKK6 | p53 | |
| CREB | JNK1 | MSK2 | p70 S6K | |
| ERK1 | JNK2 | p38 alpha | RSK1 | |
| ARY003B: human phospho-kinase | ||||
| 2×4 membranes | ||||
| Sample Type: cell lysate | ||||
| Akt 1/2/3 (S473) | eNOS (S1177) | JNK 1/2/3 (T183/Y185, T221/Y223) | p70 S6 Kinase (T421/S424) | STAT3 (Y705) |
| Akt 1/2/3 (T308) | ERK1/2 (T202/Y204, T185/Y187) | Lck (Y394) | PDGF R beta (Y751) | STAT5a (Y699) |
| AMPK alpha1 (T183) | FAK (Y397) | Lyn (Y397) | PLC gamma-1 (Y783) | STAT5a/b (Y699) |
| AMPK alpha2 (T172) | Fgr (Y412) | MSK1/2 (S376/S360) | PRAS40 (T246) | STAT5b (Y699) |
| beta-Catenin | Fyn (Y420) | p27 (T198) | Pyk2 (Y402) | STAT6 (Y641) |
| Chk-2 (T68) | GSK-3 alpha/beta (S21/S9) | p38 alpha (T180/Y182) | RSK1/2/3 (S380) | TOR (S2448) |
| c-Jun (S63) | Hck (Y411) | p53 (S15) | Src (Y419) | WNK-1 (T60) |
| CREB (S133) | HSP27 (S78/S82) | p53 (S392) | STAT2 (Y689) | Yes (Y426) |
| EGF R (Y1068) | HSP60 | p53 (S46) | STAT3 (S727) | |
| ARY004B: human phospho-immunoreceptor | ||||
| 1×4 membranes | ||||
| Sample Type: cell lysate | ||||
| 2B4/SLAMF4 | CRACC/SLAMF7 | ILT2/CD85j | LMIR6/CD300LE | Siglec-2/CD22 |
| BLAME/SLAMF8 | CTLA-4/CD152 | ILT3/CD85k | MDL-1/CLEC5A | Siglec-3/CD33 |
| BTLA | DCIR/CLEC4A | ILT4/CD85d | NKp30/NCR3 | Siglec-5 |
| CD3 epsilon | Dectin-1/CLEC7A | ILT5/CD85a | NKp44/NCR2 | Siglec-7 |
| CD5 | DNAM-1 | ILT6/CD85e | NKp46/NCR1 | Siglec-9 |
| CD6 | Fc epsilon RII/CD23 | Integrin beta 3/CD61 | NKp80/KLRF1 | Siglec-10 |
| CD28 | Fc gamma RIIA | KIR2DL4 | NTB-A/SLAMF6 | SIRP-beta 1 |
| CD84/SLAMF5 | Fc gamma RIIIA/B | LAIR-1 | PD-1 | SLAM/CD150 |
| CD229/SLAMF3 | FcRH1/IRTA5 | LAIR-2 | PECAM/CD31 | TREM-1 |
| CEACAM-1 | FcRH2/IRTA4 | LMIR1/CD300A | SHIP-1 | TREM-2 |
| CLEC-1 | FcRH4/IRTA1 | LMIR2/CD300C | SHP-1 | TREML1/TLT-1 |
| CLEC-2 | FcRH5/IRTA2 | LMIR3/CD300LF | SHP-2 | |
| ARY005: human cytokine | ||||
| 1×4 membranes | ||||
| Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma | ||||
| C5a | IL-1 alpha | IL-10 | IL-32 alpha | CXCL12/SDF-1 |
| CD40 ligand | IL-1 beta | IL-12 p70 | CXCL10/IP-10 | Serpin E1/PAI-1 |
| G-CSF | IL-1ra | IL-13 | CXCL11/I-TAC | TNF-alpha |
| GM-CSF | IL-2 | IL-16 | CCL2/MCP-1 | TREM-1 |
| CXCL1/GRO alpha | IL-4 | IL-17 | MIF | |
| CCL1/I-309 | IL-5 | IL-17E | CCL3/MIP-1 alpha | |
| ICAM-1 | IL-6 | IL-23 | CCL4/MIP-1 beta | |
| IFN-gamma | IL-8 | IL-27 | CCL5/RANTES | |
| ARY006: mouse cytokine | ||||
| 1×4 membranes | ||||
| Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma | ||||
| CXCL13/BLC/BCA-1 | IL-1 alpha | IL-10 | CXCL11/I-TAC | CXCL2/MIP-2 |
| C5a | IL-1 beta | IL-12 p70 | CXCL1/KC | CCL5/RANTES |
| G-CSF | IL-1ra | IL-13 | M-CSF | CXCL12/SDF-1 |
| GM-CSF | IL-2 | IL-16 | CCL2/JE/MCP-1 | CCL17/TARC |
| CCL1/I-309 | IL-3 | IL-17 | CCL12/MCP-5 | TIMP-1 |
| CCL11/Eotaxin | IL-5 | IL-23 | CXCL9/MIG | TNF-alpha |
| ICAM-1 | IL-6 | IL-27 | CCL3/MIP-1 alpha | TREM-1 |
| IFN-gamma | IL-7 | CXCL10/IP-10 | CCL4/MIP-1 beta | |
| ARY007: human angiogenesis | ||||
| 1×4 membranes | ||||
| Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma, saliva | ||||
| Activin A | EGF | HB-EGF | MMP-8 | Serpin B5/Maspin |
| ADAMTS-1 | EG-VEGF | HGF | MMP-9 | Serpin E1/PAI-1 |
| Angiogenin | Endoglin/CD105 | IGFBP-1 | NRG1-beta 1 | Serpin F1/PEDF |
| Angiopoietin-1 | Endostatin/Collagen XVIII | IGFBP-2 | Pentraxin 3 | TIMP-1 |
| Angiopoietin-2 | Endothelin-1 | IGFBP-3 | PD-ECGF | TIMP-4 |
| Angiostatin/Plasminogen | FGF acidic | IL-1 beta | PDGF-AA | Thrombospondin-1 |
| Amphiregulin | FGF basic | CXCL8/IL-8 | PDGF-AB/PDGF-BB | Thrombospondin-2 |
| Artemin | FGF-4 | LAP (TGF-beta 1) | Persephin | uPA |
| Tissue Factor/Factor III | FGF-7/KGF | Leptin | CXCL4/PF4 | Vasohibin |
| CXCL16 | GDNF | CCL2/MCP-1 | PlGF | VEGF |
| DPPIV/CD26 | GM-CSF | CCL3/MIP-1 alpha | Prolactin | VEGF-C |
▍订货信息：
| 货号 | 产品名称 | 规格 |
| ARY001B | Proteome Profiler Human Phospho-RTK Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY002B | Proteome Profiler Human Phospho-MAPK Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY003B | Proteome Profiler Human Phospho-Kinase Array Kit (1 KT) | 1 Kit(4套，每套2张膜PartA+PartB） |
| ARY004B | Proteome Profiler Human Phospho-Immunoreceptor Array Kit,NEW (1 KT) | 1 Kit(4张膜） |
| ARY005B | Proteome Profiler Human Cytokine Array Kit, Panel A (1 KT) | 1 Kit(4张膜） |
| ARY006 | Proteome Profiler Mouse Cytokine Array Kit, Panel A (1 KT) | 1 Kit(4张膜） |
| ARY007 | Proteome Profiler Human Angiogenesis Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY008 | Proteome Profiler Rat Cytokine Array Kit, Panel A (1 KT) | 1 Kit(4张膜） |
| ARY009 | Proteome Profiler Human Apoptosis Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY010 | Proteome Profiler Human Pluripotent Stem Cell Array Kit (1 KT) | 1 Kit(8张膜） |
| ARY011 | Proteome Profiler Human sReceptor Array, Hematopoietic (1 KT) | 1 Kit(4张膜） |
| ARY012 | Proteome Profiler Human sReceptor Array, Non-hematopoietic (1 KT) | 1 Kit(4张膜） |
| ARY013 | Proteome Profiler Mouse Adipokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY014 | Proteome Profiler Mouse Phospho-RTK Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY015 | Proteome Profiler Mouse Angiogenesis Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY016 | Proteome Profiler Rat Adipokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY017 | Proteome Profiler Human Chemokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY018 | Proteome Profiler Human Cell Stress Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY019 | Proteome Profiler Human Kidney Biomarker Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY020 | Proteome Profiler Mouse Chemokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY021B | Proteome Profiler Human Protease Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY022 | Proteome Profiler Human XL Cytokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY023 | Proteome Profiler Human Protease Inhibitor Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY024 | Proteome Profiler Human Adipokine Array Kit (1 KT) | 1 Kit(4张膜） |
| ARY025 | Proteome Profiler Human Protease/Protease Inhibitor Array (1 KT) | 1 Kit(4张膜） |
| ARY026 | Proteome Profiler Human XL Oncology Array (1 KT) | 1 Kit(4张膜） |
| ARY027 | Proteome Profiler Human Ubiquitin Array (1 KT) | 1 Kit(4张膜） |
| ARY028 | Proteome Profiler Mouse XL Cytokine Array (1 KT) | 1 Kit(4张膜） |
| ARY029 | Proteome Profiler Human NFkB Pathway Array Kit (1 KT) | 1 Kit(4张膜） |
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