Application of SMALP in Screening of Different Membrane Proteins
2024-11-19 来源:本站 点击次数:252
Description
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
Datasheets
Citations
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
Datasheets
| FEATURE | |
| State | Lyophilized Powder, to be solved with water |
| Polymer styrene-to-maleic anhydride ratio | 2:1 |
| Molecular weight | 6,500 Da |
| pH after solving | 7.5 |
| Solubility | 40 % |
| Divalent cationic tolerance | < 5 mM |
| Color | Yellowish |
| Odor | Odorless |
| Freeze-thaw stable? | Yes |
| Shipping Temperature | ambient temperature |
| Storage of lyophilized copolymer | -20°C for several years |
| Storage of dissolved copolymer | 2-8°C for several days |
| Other noteworthy feature | Most commonly used SMA |
| Structure |  |
Citations
| STABILIZED PROTEIN | YEAR | AUTHOR |
| Bacterial Membrane Proteins | 2022 | Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L. |
| - | 2023 | Hernandez L.M.R. & Levental I. |
| Alpha-Toxin degrading proteins | 2023 | Sun L., Wang D., Noh R., Fang H., Gao W., Zhang L. |
| ATP-binding cassette (ABC) transporter | 2023 | Dimitrova V.A., Song S., Karagiaridi A., Marand A., Pinkett H.W. |
| Env-protein of HIV | 2023 | Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C., Smith III. A.B.S, Sodroski J.G. |
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
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