| Description: |
Adiponectin is an adipose-derived secreted protein containing 236 amino acid residues. It |
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is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma |
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protein.The circulating levels of adiponectin are decreased under conditions of obesity, |
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insulin resistance,and type II diabetes. Disruption of adiponectin in mice causes insulin |
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resistance and neointimal formation.Conversely, administration of recombinant adiponectin |
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suppresses hepatic glucose production,and reverses insulin resistance associated with |
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both lipoatrophy and obesity.The protective role of adiponectin is attributed to its anti- |
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inflammatory properties (e.g. ability to suppress expression of TNF-α and class A |
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scavenger receptor in macrophages) Recombinant adiponectin is a multimeric glycoprotein |
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containing amino acids Glu-19 to Asn- 244 of the adiponectin precursor protein fused to an |
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N- terminal histidine tag.Monomeric glycosylated adiponectin migrates at an apparent |
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molecular weight of approximately 35.0 kDa by SDS PAGE analysis under |
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reducing conditions. |
