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重组链霉亲和素/Streptavidin (r-SA)生产技术

2019-09-16     来源:本站     点击次数:100

重组链霉亲和素/Streptavidin (r-SA)

Recombinant Streptavidin (r-SA)

 Product Name: Recombinant Streptavidin (r-SA)

Packing Details: 1 mg, 10 mg, 100 mg, 500 mg

Formulation: Lyophilized from 5 mM PB, pH 6.3

Mol. Wt.: 16.5 kDa

Resources: Escherichia coli (E. coli)

Purity: ≥95% by SDS-PAGE analysis

Activity: ≥13 U/mg protein (Determined by Youngshe according to modified Green Method)

Storage Condition: -20 oC

Storage Duration: 3 years

Description:

Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore it has a reducing nonspecific binding level as compared to avidin. Streptavidin has been extensively applied in various biological fields, such as ELISA, IHC, TRFIA, quantifying PCR, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well.

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