Preparation :

Produced in C3H/HeJ mice using highly pure (>98%) recombinant human MCP-3 as the immunizing antigen. This IgG1_K antibody was purified from ascites fluid by Protein A affinity chromatography.

Catalog #:

500-M73

Source :

Mice

Formulation :

A sterile filtered antibody solution was lyophilized from PBS.

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^o C. Frozen aliquots are stable for at least six weeks at 2-8^o C or for at least six months at -20^o C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Neutralization 

To yield one-half maximal inhibition [ND ₅₀ ] of the biological activity of hMCP-3 (100 ng/ml), a concentration of 1.0-3.0 μg/ml of this antibody is required.

Elisa 

In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human MCP-3 when used with 's biotinylated antigen affinity purified anti-human MCP-3 (500-P37Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

Western Blot 

To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Country of Origin :

USA

Special Sizes:
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.