英国OGT牛津基因 aCGH芯片(安捷伦定制)
英国牛津基因OGT(Oxford Gene Technology)是由Edwin Southern教授于1995年创立,Southern教授是Southern blot印记杂交方法的发明人(1975年)。OGT产品具有三方面的独特之处:独特探针设计并结果可靠的aCGH芯片、高效率的标记试剂盒,无需Digest基因组DNA,简化实验步骤,缩短时间并保证了极低的DLR值和更高的信噪比、全球唯一具备interpret功能强大并简单易用的软件,软件直接注释出每一个报告的畸变所对应的基因和相关综合征的详细信息并直接连接到相关数据库,可以很方便的进行分类。
OGT的aCGH芯片是安捷伦的定制芯片,可以在Agilent和AXON的芯片扫描仪上进行扫描,扫描分辨率要求:8x60k为2um或3um,8x15k为5um。得出的Feature Extraction之后的数据用OGT软件进行分析,同时OGT也可以将安捷伦aCGH的数据进行转换后,利用OGT软件处理数据并给出结果。
芯片特点:
芯片检测流程:
选择OGT基因芯片的理由:
理由一: CytoSure aCGH芯片流程省去基因组DNA Digestion,并极大减少人工操作时间。
CytoSure CNV+SNP芯片流程(左)与传统SNP流程(右)对比,CytoSure CNV+SNP芯片流程省去基因组DNA Digestion,并极大减少人工操作时间。
理由二:节省成本,不需要文库质控
理由三:CytoSure软件快速给出所发现的畸变的临床意义
CytoSureTM解析软件优势
CytoSureTM解析软件是一款强大的aCGH数据分析软件,且简单易学。软件包括标准工作流程和用户自定义工作流程两种模式,标准工作流程可自动化完成数据分析,极大减少用户干预;自定义工作流程可允许用户根据自身实验要求自行设定分析流程。该软件随CytoSure aCGH芯片免费且独家提供。
CytoSureTM解析软件优势如下:
CytoSure Interpret Software全面制定的跟踪链接简化了畸变结果的注释过程
CytoSure解析软件的自动SNP和LOH检测。暗红色矩形表示LOH区域。绿色和亮红色矩形分别表示扩增和缺失。图中显示相同的CLL样品,并清楚地示出p13.31-p12.3的p臂端粒区域和LOH区域的扩增。
CytoSure Interpret Software全自动染色体异常检测清晰显示染色体异常:癌症基因普查基因跟踪可轻松检测到慢性淋巴细胞性白血病(CLL)样品的12号染色体中包含锌脂蛋白基因ZP384。
应用方面:
产品订购信息:
Genome-wide Arrays |
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Product | Contents | Cat.no |
CytoSure ISCA v2 (4x44k) | Microarray with four arrays of 44,000 spots; CytoSure Interpret Software | 020042 |
CytoSure ISCA v2 (8x60k) | Microarray with eight arrays of 60,000 spots, featuring spike-in control; CytoSure Interpret Software | 020040 |
CytoSure ISCA v2 (4x180k) | Microarray with four arrays of 180,000 spots, featuring spike-in control; CytoSure Interpret Software | 020041 |
CytoSure ISCA +SNP(4x180k) | Microarray with four arrays of 180,000 spots;CytoSure Interpret Software | 020051 |
CytoSure ISCA UPD (4x180k)* | Microarray with four arrays of 180,000 spots; CytoSure Interpret Software | 020050 |
CytoSure Syndrome Plus v2 (2x105k) | Microarray with two arrays of 105,000 spots;CytoSure Interpret Software | 020019 |
CytoSure Aneuploidy (8 x 15k)* | Microarray with eight arrays of 15,000 spots;CytoSure Interpret Software | 020024 |
CytoSure Single Cell Aneuploidy (8 x 15k) | Microarray with eight arrays of 15,000 spots optimised for research usingamplified DNA | 020043 |
Gene-focused arrays |
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product | content | cat.no |
CytoSure Molecular Testing Array A (8x60k)* | Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software. Content focus: neuromuscular disorders. | 020062 |
CytoSure Molecular Testing Array B (4x180k) | Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Content focus: in-born metabolic disorders | 020061 |
CytoSure Molecular Testing Array C (4x180k) | Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Content focus: mental retardation and autism | 020060 |
CytoSure Custom Molecular Testing Array (various formats) | Microarray with a choice of formats; CytoSure Interpret Software | 020018 |
CytoSure DMD (4x44k)* | Microarray with four arrays of 44,000 spots; CytoSure Interpret Software | 020023 |
CytoSure Cancer +SNP array (4x180k) | Microarray with four arrays of 180,000 spots; CytoSure Interpret Software | 700090 |
CytoSure Haematological Cancer +SNP(8x60k) | Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software | 020070 |
Chromosome-specific arrays |
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Product | Contents | Cat.no |
Chromosome X (4x44k) | Microarray with four arrays of 44,000 spots; CytoSure Interpret Software | 020015 |
Chromosome X (2x105k) | Microarray with four arrays of 105,000 spots; CytoSure Interpret Software | 020021 |
Custom Designed aCGH Arrays |
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Product | Contents | Cat.no |
CytoSure Custom Array | Microarray with a choice of formats; CytoSure Interpret software | 020018 |
CytoSure™ Interpret Software |
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Product | Contents | Cat.no |
CytoSure Interpret Software | Class-leading microarray analysis software. Complimentary with all CytoSure arrays | 020022 |
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