精子计数板(Petroff-Hausser Counting Chamber)：或者称细菌计数板Bacteria Counter，具有单一计数区，盖玻片表面被激光蚀刻有计数网格，改良牛鲍氏型，深度：0.02mm，这个优化的深度形成的单层精子细胞。用于细菌或者用于未稀释标本的精子计数，计数精子时一般要杀死精子或者固定精子后进行。

Directions for Use:

In order to count the number of sperm per milliliter, dilution is not necessary. To immobilize sperm, there are two recommendations:

1. Add a drop of Formaldehyde
   OR

2. Run semen under hot water, which will kill or immobilize.

Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.02 mm below the cover glass, so that the volume over a square millimeter is 0.02 cubic mm. Count all cells within this center square millimeter (1mm x 1mm area).

Neubauer Ruling

The number of sperm per milliliter (ml) = Number of cells counted X dilution (if used) X 50,000*

*50,000 = 50 (cell depth is 1/50) X 1000 (1000 cubic mm = 1 milliliter)

Cleaning:

After completing the count, remove the cover glass and clean the counting chamber with water or a mild cleaning solution (10% solution of bleach). Dry the counting chamber with a soft cloth or wipe, or rinse with acetone.