30mm玻璃培养皿 WILLCOWELL GWST-5030--性能参数，报价/价格，图片

基本信息

产品名称：

30mm玻璃培养皿 WILLCOWELL GWST-5030

英文名称：

WILLCOWELLS

国产/进口：

进口

产地/品牌：

荷兰WILLCOWELLS

型号：

GWST-5030

参考报价：

6600

销售商：

上海意果科技有限公司

总点击数：

69433

更新日期：

2025-05-28

产品类别：

其他实验耗材

性能参数

参考编号： GWST-5030

包装

120

尺寸/毫米

50x7 毫米。

底部

玻璃 D-263 M

光圈

30毫米。

体积/微升

1100 μL。

通风口

不

吸塑包装

PET-G BLUE MED泡罩包装，带 Tyvek® 盖；“单件”包装。
每泡罩包装 4 个培养皿 x 30 个泡罩包装 = 每箱 120 个培养皿。
经伽马射线辐射无菌，最低 10 kGy。
注意：产品在包装打开或损坏之前都是无菌的。

无菌

5年。
平整度：

谈到玻璃底盘时，有两种“平整度”的定义。

首先，是盘的玻璃盖玻片底部的平整度，玻璃盖玻片与盘粘合在一起，当放置在加热台上时，它绝对水平（- 并且应该与加热台齐平；只有这样，才没有空气缓冲！）。
玻璃盖玻片水平：
平放或水平放置意味着，当您将加热台移动到另一个位置时，显微镜的光线正交穿过玻璃底部（非常重要），并且您的细胞保持聚焦（例如但不限于：时间推移显微镜）。
玻璃盖玻片与载物台“齐平”：
当玻璃盖玻片与载物台齐平时，这意味着玻璃和加热台之间没有空气缓冲。WillCo -dish ® 设计是第一个采用这种特殊设计的培养皿。这就是使 WillCo-dish ®DESIGN 具有如此独特的性质。此功能也非常重要，因为它确保了从加热阶段到培养皿内的培养基/液体的热量均匀分布。
所有细胞都将均匀受热，这对您宝贵的细胞的健康和工作的成功至关重要！
注意：如果不是这种情况，如果在加热台和玻璃盖玻片底部之间有空气缓冲器，则热量将不均匀地分布到您的细胞上。它们的发育将停止，它们的寿命将缩短，这将极大地影响您的工作结果。
盖玻片粗糙度：
其次，在讨论玻璃盖玻片的“平整度”时，我们指的是玻璃表面的平整度 (Ra) 或粗糙度。我们提供两种规格：表面平整度 Ra 0.005 毫米（标准）和 Ra 0.01 毫米，后者表面粗糙度是后者的两倍。
我们仅使用特选玻璃，厚度 #1.5H，170 微米玻璃 +/- 5 微米，以及 Ra 0.005 毫米的平整度，用于我们所有的标准生产。

您可能需要玻璃底培养皿涂上涂层，以便细胞更好地粘附。我们不对
WillCo-dish ®的玻璃进行涂层处理，因为在我们生产 WillCo-dish ® 的高标准洁净室中不允许使用液体
。此外，我们很少收到有关涂层玻璃的询问。

在过去的二十年里，迄今为止，我们大多数备受尊敬的WillCo -dish ®用户都自己保养涂层，
这就是我们为您提供以下涂层程序的原因。我们欢迎您的建议，以进一步支持您的
同事。

玻璃盖玻片的涂层程序
1.：胶原蛋白，
2.：聚赖氨酸和聚鸟氨酸，
3.：纤连蛋白，
4.：层粘连蛋白。
1 .：胶原蛋白
胶原蛋白可用于涂覆玻璃盖玻片，以促进上皮细胞、内皮细胞和肌肉细胞、神经元、PC12
和 CHO 细胞系的生长。
I 型胶原蛋白最常用于涂覆细胞培养基质，因为它很容易从鼠尾中获得。对于
短期培养，只需将胶原蛋白涂在玻璃盖玻片上并晾干即可。
1 . 用 30% 乙醇将胶原蛋白溶液稀释 1:10 - 1:50，然后铺在无菌玻璃盖玻片表面。
2 . 在组织培养罩内风干。
3 . 细胞可直接接种在胶原蛋白表面。
4 . 以这种方式制备的胶原蛋白涂层在长期培养中往往会从玻璃上脱落。
胶原蛋白 IV是基底膜的主要成分，因此是培养
许多细胞类型的更生理性的涂层。
对于长期培养，胶原蛋白 I 和 IV 可以先用聚赖氨酸或
聚鸟氨酸涂覆玻璃，然后涂在玻璃盖玻片上。这提供了更稳定的胶原蛋白涂层。
1 . 在 0.15 M 硼酸盐缓冲液（pH 8.3）中制备 0.1-1 mg/ml 的聚赖氨酸或聚鸟氨酸（分子量为 30,000 - 70,000）。
过滤除菌。
2 . 加入足够的溶液，使其覆盖在无菌玻璃盖玻片的表面上。
3 . 在室温下孵育 2-24 小时。
4 . 吸出溶液，用水清洗盖玻片 3 次。
5 . 将胶原蛋白溶液（100 ug/ml 的水溶液）覆盖在盖玻片的表面上。
6 . 孵育 4 - 16 小时。
7 . 用培养基冲洗一次，并接种细胞。
或者，对于长期培养，双层胶原蛋白涂层可以提供稳定的涂层。
1 . 在无菌玻璃盖玻片上滴几滴无菌胶原蛋白 I 溶液。
2 . 立即将盖玻片放入装有用浓氢氧化铵润湿的滤纸的盖玻片中，
用氢氧化铵蒸气中和 2 分钟。这将导致
胶原蛋白凝胶化。
3 . 用无菌水清洗盖玻片两次。
4 . 在凝胶化的胶原蛋白表面轻轻滴几滴胶原蛋白，然后风干。
5 . 在几小时内用于细胞培养。
明胶也可用于培养某些细胞类型，包括神经胶质细胞。
1 . 将 100 毫克明胶溶解于 100 毫升水中（三重玻璃蒸馏水或 RO）。
2 . 高压灭菌。
3 . 趁热充分混合明胶溶液。
4 . 加入足够的溶液，使其覆盖无菌玻璃盖玻片表面。
5 . 在 4oC 下冷藏 2-24 小时。
6 . 抽吸去除明胶并加入无菌水。
7 . 培养皿可在 4oC 下保存长达一周。
在用于细胞培养前立即倒掉水
2 .：聚赖氨酸和聚鸟氨酸：
几乎所有类型的细胞都粘附在这些碱性氨基酸聚合物上。它们特别适用于中枢神经系统
神经元的培养。L-
或 D- 异构体可用于细胞附着，但 D- 异构体可能更受青睐，因为它不易被
细胞释放的蛋白酶分解。
1 . 在 0.15 M 硼酸盐缓冲液（pH 8.3）中制备 0.1-1 mg/ml 的聚赖氨酸或聚鸟氨酸（分子量为 30,000 - 70,000）。过滤
除菌。
2 . 加入足够的溶液以覆盖无菌玻璃盖玻片表面。
3 . 在室温下孵育 2-24 小时。
4 . 吸出溶液并用培养基或 PBS 清洗盖玻片 3 次。
5 . 立即加入细胞悬浮液或生长培养基。
3.：纤连蛋白：
纤连蛋白是一种细胞外基质成分，用于培养内皮细胞、成纤维细胞、神经元和 CHO 细胞。1
. 可通过将 1 mg/ml 纤连蛋白溶解在 PBS 中来制备原液。过滤除菌并分装冷冻。2
. 在基础培养基或 PBS 中将原液稀释至 50-100 ug/ml。3
. 加入足够的溶液以覆盖无菌玻璃盖玻片表面。4
. 在室温下孵育 30-45 分钟。5
. 抽吸以去除纤连蛋白并用培养基或 PBS 冲洗盖玻片。6
. 立即加入细胞悬浮液或生长培养基。不要让涂层变干。
4.：层粘连蛋白：
层粘连蛋白是一种细胞外基质成分，用于培养神经元、上皮细胞、白细胞、成肌细胞和
CHO 细胞。
1 . 可通过将 1 mg/ml 层粘连蛋白溶解在 PBS 中来制备原液。过滤除菌，分装冷冻。
2 . 在基础培养基或 PBS 中将原液稀释至 10-100 ug/ml。
3 . 加入足够的溶液，使其覆盖在无菌玻璃盖玻片表面。
4 . 在室温下孵育数小时。
5 . 抽吸以去除层粘连蛋白，并用培养基或 PBS 冲洗盖玻片。
6 . 立即加入细胞悬浮液或生长培养基。不要让涂层变干。
7 . 先用聚赖氨酸或聚鸟氨酸涂覆玻璃盖玻片，然后用层粘连蛋白涂覆，可以增加
使用此方法施加的层粘连蛋白的浓度。

Ref. Code:GWST-5030

Unit

120

Size / mm

50x7 mm.

Bottom

Glass D-263 M

Aperture

30 mm.

Vol / μL

1100 μL.

Vents

No.

BLISTER-PACK

:   PET-G BLUE MED blister with Tyvek® cover; 'Single unit' packed.
       4 Dishes per blister x 30 blisters = 120 Units per case.
       STERILE R by Gamma irradiation, 10 kGy minimum.
       NOTE: Products are sterile, until packaging is opened or damaged.

STERILE R

5 Years.
Flatness:

When speaking about Glass Bottom Dishes, there are two definitions of 'flatness'.

First, there is the flatness of the glass coverslip bottom of the dish, where the glass coverslip is bonded to the dish as such, that it is absolutely horizontal when positioned on the warming-stage (- and should be flush with the warming-stage; only then, there is no air buffer!).
Glass coverslip horizontal:
Positioned flat or horizontal means, that the light of the microscope passes through the glass bottom orthogonally (very important) and that your cells stay in focus, when you move the warming-stage to another position (E.g. but not limited to: Time Laps Microscopy).
Glass coverslip 'flush', with the stage:
When the glass coverslip is flush with the stage, it means that there is no air-buffer, between the glass and the warming-stage. The WillCo-dish® design, was - and is, the first dish to have this exceptional design. That is what makes the WillCo-dish®DESIGN of such an unique nature. This feature too is very important, because it ensures an even distribution of the heat, from the warming-stage to the media/liquid, inside the dish.
All cells will be heated evenly, essential for the well being of your precious cells and for the success of your work!
Note: If this is not the case, if there is an air-buffer between the warming-stage and the bottom of the glass coverslip, there will be an uneven distribution of the heat to your cells. They will arrest in their development, their life span will shorten and it will influence the results of your work, dramatically.
Coverslip roughness:
Secondly, when discussing 'flatness' of the glass coverslip, we mean the surface flatness (Ra) or roughness of the surface of the glass. We offer two specifications; surface flatness Ra 0.005 mm. (standard) and Ra 0.01 mm., which surface is twice as rough.
We use specially selected glass only, thickness #1.5H, 170 micron glass +/- 5 micron, as well as this Ra 0.005 mm. flatness, for all our standard production.

You may need your Glass Bottom dishes to have a coating, for better adherence of your cells. We do
not administer coatings on the glass of our WillCo-dish®es, because it is not allowed to use liquids in the high standard
clean room, where we manufacture our WillCo-dish®es. Besides, we only rarely received an inquiry, for coated glass.

Over the past twenty years, by far the most of our well respected WillCo-dish®USERS, took care of their own coatings,
the reason why we offer you some coating procedures, below. We welcome your suggestions, to further support your
Colleagues.

Coating procedures for glass coverslips
1.: COLLAGEN,
2.: POLYLYSINE and POLYORNITHINE,
3.: FIBRONECTIN,
4.: LAMININ.

1.: COLLAGEN
Collagen may be used to coat glass coverslips for the growth of epithelial, endothelial and muscle cells, neurons, PC12
and CHO cell lines.
Type I collagen is most often used for coating substrates for cell culture, because it is easily obtainable from rat tails. For
short term cultures, collagen can be simply applied to glass coverslips and allowed to dry.
1. Dilute collagen solution 1:10 - 1:50 with 30% ethanol and spread over surface of sterile glass coverslip.
2. Air dry in a tissue culture hood.
3. Cells can be seeded directly on the collagen surface.
4. Collagen coating prepared in this way tends to detach from the glass in long-term cultures.
Collagen IV is the major constituent of basement membrane and is therefore a more physiological coating for the culture
of many cell types.
For long-term cultures, collagen I and IV can be applied to glass coverslips by first coating the glass with polylysine or
polyornithine. This provides a more stable collagen coating.
1. Prepare polylysine or polyornithine (MW of 30,000 - 70,000) at 0.1-1 mg/ml in 0.15 M borate buffer
(pH 8.3). Filter sterilize.
2. Add enough solution to pool over surface of sterile glass coverslip.
3. Incubate 2-24 hours at room temperature.
4. Aspirate solution and wash coverslips 3 times with water.
5. Pool collagen solution, 100 ug/ml in water over surface of coverslip.
6. Incubate 4 - 16 hours.
7. Rinse once with media and seed with cells.
Alternatively, for long-term cultures, double layered collagen coatings can provide a stable coating.
1. Spread a couple of drops of sterile collagen I solution on the sterile glass coverslip.
2. Immediately neutralize for 2 minutes with ammonium hydroxide vapors by placing the dish of coverslips
in a covered dish containing filter paper wet with concentrated ammonium hydroxide. This will cause
the collagen to gel.
3. Wash coverslips twice with sterile water.
4. Gently spread a couple of drops of collagen over the surface of the gelled collagen and air dry.
5. Use within a few hours for cell culture.
Gelatin can also be used for the culture of some cell types including glial cells.
1. Dissolve 100 mg gelatin in 100 ml water (triple glass distilled or RO).
2. Autoclave to sterilize.
3. While hot, thoroughly mix gelatin solution.
4. Add enough solution to pool over surface of sterile glass coverslip.
5. Chill for 2-24 hours at 4oC.
6. Remove gelatin by aspiration and add sterile water.
7. Dishes can be stored for up to one week at 4oC.
Remove water immediately before use for cell culture
2.: POLYLYSINE AND POLYORNITHINE:
Nearly all types of cells adhere to these polymers of basic amino acids. They are particularly useful for the culture of CNS
neurons.
The L- or D-isomers can be used for cell attachment, however, the D-isomer may be preferred because it is not subject to
breakdown by proteases released by cells.
1. Prepare polylysine or polyornithine (MW of 30,000 - 70,000) at 0.1-1 mg/ml in 0.15 M borate buffer (pH 8.3). Filter
sterilize.
2. Add enough solution to pool over surface of sterile glass coverslip.
3. Incubate 2-24 hours at room temperature.
4. Aspirate solution and wash coverslips 3 times with media or PBS.
5. Immediately add cell suspension or growth media.
3.: FIBRONECTIN:
Fibronectin is an extracellular matrix constituent use for the culture of endothelial cells, fibroblasts, neurons and CHO cells.
1. Stock solution can be prepared by dissolving 1 mg/ml fibronectin in PBS. Filter sterilize and freeze in aliquots.
2. Diluted stock solution to 50-100 ug/ml in basal medium or PBS.
3. Add enough solution to pool over surface of sterile glass coverslip.
4. Incubate for 30-45 min at room temperature.
5. Aspirate to remove fibronectin and rinse coverslips with media or PBS.
6. Immediately add cell suspension or growth media. Do not allow coating to dry.
4.: LAMININ:
Laminin is an extracellular matrix constituent used for the culture of neurons, epithelial cells, leukocytes, myoblasts and
CHO cells.
1. Stock solution can be prepared by dissolving 1 mg/ml laminin in PBS. Filter sterilize and freeze in aliquots.
2. Diluted stock solution to 10-100 ug/ml in basal medium or PBS.
3. Add enough solution to pool over surface of sterile glass coverslip.
4. Incubate several hours at room temperature.
5. Aspirate to remove laminin and rinse coverslips with media or PBS.
6. Immediately add cell suspension or growth media. Do not allow coating to dry.
7. Coating the glass coverslip first with polylysine or polyornithine and then laminin may increase the
concentration of laminin applied using this method.

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