Anti-P38 MAPK (Phospho-Thr180)
Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
p38 MAPK around the phosphorylation site of threonine 180 (E-M-TP-G-Y ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificity/Sensitivity:
p38 MAPK (phospho-Thr180) antibody detects endogenous levels of p38 MAPK only when phosphorylated at threonine 180.
Reactivity:
Human, Mouse, Rat
Applications:
IHC: 1:50~1:100
References:
Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4(4): 1339-1346 Meng F, et al. (2005) Am J Physiol Cell Physiol May 25
Jin ZH, et al. (2005)Oncogene Mar 17; 24(12): 1973-1981