产品货号：K-TREH 

品牌：Megazyme

产品品名：海藻糖检测试剂盒

英文品名：Trehalose Assay Kit

规格型号：100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser) 

A simple method for the rapid and reliable measurement of trehalose infoods, beverages and other materials.
Suitable for manual, auto-analyser and microplate formats.

INTRODUCTION:

Trehalose is a naturally occurring disaccharide containing two glucose
molecules bound in an α,α-1,1 linkage. This structure results in a
chemically stable, non-reducing sugar with many important functional
characteristics. Trehalose is commonly found in nature, provides
a source of energy, and has been shown to be a primary factor in
stabilising organisms during times of freezing, drying and heating.
Trehalose is consumed as part of a normal diet in mushrooms, honey,
lobster, shrimp and foods produced using baker’s and brewer’s yeast.
Trehalose has been determined to be generally recognised as safe
(GRAS) as a multipurpose ingredient for all uses in food, including
as a sweetener, stabiliser, thickener and flavour enhancer, in general
accordance with current good manufacturing practices. It has a
uniquely high glass transition temperature for a disaccharide, is nonbrowning,
and will not hydrolyse at low pH or high temperatures.
This results in more stable colours and flavours in foods to which
it is added. Trehalose is 45% as sweet as sucrose when compared
in a 10% (w/v) solution and is stable at up to 94% relative humidity.
The low hygroscopic nature of trehalose dihydrate results in a freeflowing,
stable dry product. As a non-reducing sugar it does not
react with amino acids or proteins to initiate Maillard browning.

KITS:

Kits suitable for performing 100 assays in manual format (or 1100
assays in auto-analyser format or 1000 assays in microplate format)
are available from Megazyme. The kits contain the full assay method
plus:
Bottle 1: Buffer (25 mL, pH 7.0) plus magnesium chloride and
sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NADP+ plus ATP.
Stable for > 5 years at -20°C.
Bottle 3: Hexokinase plus glucose-6-phosphate
dehydrogenase suspension, 2.25 mL.
Stable for > 2 years at 4°C.
Bottle 4: Trehalase suspension (2.25 mL).
Stable for > 2 years at 4°C.
Bottle 5: D-Glucose standard solution (5 mL, 0.4 mg/mL)
in 0.2% (w/v) benzoic acid.
Stable for > 4 years at room temperature.
Bottle 6: Trehalose dihydrate control (~ 2 g).
Stable for > 4 years at room temperature.

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