产品货号：K-FRUGL 

品牌：Megazyme

产品品名：D-果糖和D-葡萄糖检测试剂盒

英文品名：D-Fructose/D-Glucose Assay Kit

规格型号：110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser) 

Enzymatic UV-method for the measurement of D-fructose and/or D-glucosein plant and food products.

INTRODUCTION:

D-Glucose and D-fructose are found in most plant products. In foods,
they are present in significant quantities in honey, wine and beer, and
a range of solid foodstuffs such as bread and pastries, chocolate and
candies. In the wine industry, the D-glucose and D-fructose content,
often referred to as total reducing sugars, is one of the most important
quality parameters and is monitored at each stage of the wine making
process. These sugars can be measured either independently (see
page 4, “A”), or if more appropriate, simultaneously (see page 7, “B”).
The procedure for auto-analyser applications is described on page 9
(“C”), and the microplate procedures for independent or simultaneous
determinations are described on pages 10 and 11 (“D”), respectvely.

KITS:

Kits suitable for performing 110 assays in manual format (or 1100 assays
in auto-analyser format or 1100 assays in microplate format) are available
from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (15 mL, pH 7.6) plus sodium azide (0.02% w/v) as
a preservative.
Stable for > 2 years at 4°C.
3
Bottle 2: NADP+, ATP and PVP.
Stable for > 5 years at -20°C.
Bottle 3: Hexokinase plus glucose-6-phosphate dehydrogenase
suspension, 2.25 mL.
Stable for > 2 years at 4°C.
Bottle 4: Phosphoglucose isomerase suspension (2.25 mL).
Stable for > 2 years at 4°C.
Bottle 5: D-Glucose plus D-fructose standard solution
(5 mL, 0.2 mg/mL of each sugar).
Stable for > 2 years at 4°C.

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