Mouse mAb to CD43b Leukosialin
Clone BRA7G
lsotype IgM
Source
A BALB/c mouse was immunized with immature pluripotential human leukemia cell line K562 and splenocytes were fused with SP2/0.Ag14 myeloma cells.
Specifications
The BRA7G antibody reacts with a 95-115 kD protein on T cells and thymocytes and a 115-135 kD molecule on neutrophils and platelets Due to many Ser and Thr residues it is heavily glycosylated with about 70-85 O-linked oligosaccharides. It is a mucin-like
Applications
CD43 is present on many lymphoid cells like thymocytes, T cells, granulocytes, monocytes, macrophages, NK cells, platelets, activated B cells, plasma cells and on bone marrow hematopoietic stem cells. BRA7G reacts very well in Flow cytometry but also on p
Frozen section +
Paraffin sections
Flow cytometry +
ELISA
Blocking
Immuno-precipitation
Western blotting +
References
This Clone : Chorvath et al., Neoplasma 39(6), 1992 Leukoeyte Typing V (in press). General : Pailant et al., PNAS 86, 1328-1332, 1989. Shelley et al., Biochem. J. 270, 569-576, 1990. Barclay et al. The Leucocyte Antigen Factsbook, Acad. Press,