Mouse mAb to CD44 H-CAM
Clone 156-3C11
lsotype Mouse IgG2a
Source
A BALB/c mouse was immunized with stimulated human leucocytes. Splenocytes were fused with mouse myeloma NS1 cells.
Specifications
The mAb 156-3C11 reacts with CD44, a glycoprotein with various molecular weight (29-3 7 kD on haematopoietic cells and 5 1 kD on epithelia cells. CD44 has a cell-cell and cell-matrix adhesion function and is linked to the cytoskeleton. The mAb was cluster
Applications
The mAb has been applied in flow cytometry and can be used for paraffin staining of haematopoietic cells and many non haematopoietic cells including a wide variety of tumor cells. The mAb selectively interfere with lymphocyte binding to lymph node, mucosa
Frozen section +
Paraffin sections +
Flow cytometry +
ELISA n.d.
Blocking
Immuno-precipitation n.d.
Western blotting n.d.
References
Schlossman et al., Leukocyte typing V, Oxford Univ. Press, 1713-1719, 1955, (1993).