重组人促红细胞生成素-beta(EPO-b)
Recombinant Human Erythropoietin-beta Certificate of Analysis and Data Sheet |
| Description : | |
| Recombinant Human EPO-beta is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 30,400 Dalton. Recombinant Human Erythropoietin-beta is purified by proprietary chromatographic techniques. | |
| Physical Appearance: | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation: | |
| Each mg of lyophlilzed powder contains 2.3mg Sodium phosphate dibasic, 0.55mg sodium phosphate monobasic buffer, 5.8mg sodium chloride and 0.06mg calcium chloride and 0.004% tween 20. | |
| Solubility: | |
| It is recommended to reconstitute the lyophilized EPO-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: | |
| Lyophilized EPO-beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution EPO-b should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
| Purity: | |
| Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Dimers and aggregates: | |
| Less than 1% as determined by silver stained SDS-PAGE gel analysis | |
| Biological Activity: | |
| ProSpec’s EPO-b is fully biologically active when compared to standard. The Specific Activity was measured by Normocyth-aemic mice and was found to be 120,481IU/mg. | |
| Endotoxin: | |
| Less than 0.1 ng/µg (IEU/µg) of EPO-beta. | |
| Usage: | |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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