重组人白介素7(IL-7)--性能参数，报价/价格，图片

基本信息

产品名称：

重组人白介素7(IL-7)

英文名称：

国产/进口：

进口

产地/品牌：

prospec

型号：

2ug,货号:CYT-254

参考报价：

700

销售商：

北京启维益成科技有限公司试剂部

总点击数：

2602

更新日期：

2024-12-05

产品类别：

细胞生物学试剂

性能参数

Recombinant Human Interleukin-7 (IL-7)

Certificate of Analysis and Data Sheet

Source:

E.Coli

Catalog No.

CYT-254

	Background :
	Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients.
	Description :
	Recombinant Human IL-7 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 172 amino acids and having a molecular mass of 17412 Dalton. Human IL-7 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized (freeze-dried) powder.
	Formulation:
	Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized rHuIL-7 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized rHuIL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-7 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Cys-Asp-Ile.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's rHuIL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is less then 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg (IEU/µg) of rHuIL-7.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

	Gene:
	Name:IL7
	Protein synonyms/aliases:
	Interleukin-7 precursor (IL-7).
	Protein Family:
	Belongs to the IL-7/IL-9 family.

公司简介

北京启维益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由从事生物领域多年销售经验人士创立。目前我公司已经与几十家著名的试剂供应商建立了长期稳定的合作关系，已成为众多国际知名品牌的中国总代理或一级代理商；并且为满足国内客户的最新需求，我们也与时俱进地不断与世界上更多的知名品牌洽谈合作代理事宜；企业的主要目标是把国外有特点的生物前沿产品引进中国，让国内科学研究机构和制药企业尽早使用上最新型的科研产品。

我们代理的产品覆盖细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。

细胞生物学包含细胞培养，细胞迁移，细胞信号转导等。

植物学包含植物抗体，植物抗生素，植物组织培养试剂等。

分子生物学包含表观遗传试剂盒，核酸提取，转染试剂等。

免疫学包含抗体，各类检测试剂盒等。

生物化学包含各类生化试剂，化学小分子，常规试剂等。

蛋白组学包含不同种属的重组蛋白，天然提取蛋白，细胞因子等。

生物制药与诊断试剂研发方面的试剂包含各类蛋白抑制剂，酶类等。

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