Description:

Rabbit polyclonal antibody

  Background:

The H+-ATPase, a protein with a molecular mass of about 100 kD, is composed of a single polypeptide that is predicted to beanchored in the plasma membrane by 10 membrane-spanning regions. 
The proton-pump ATPase (H+-ATPase) of the plant plasma membrane acts as a primary transporter by pumping protons out of the cell, thereby creating pH and electrical potential differences across the plasmalemma. Transport of many solutes (ions, metabolites, etc.) into and out of the cell involves secondary transporters whose ability to function is directly dependent on the proton-motive force created by the H+-ATPase.

  Synonyms:

H+ATPase, AHA, HA

  Immunogen:

KLH-conjugated synthetic peptide of H+ATPase derived from Arabidopsis thaliana AT2G18960, AT4G30190, AT5G57350.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

104 / 103 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Brassica napus, Gossypium raimondii, Zea mays, Setaria viridis, Oryza sativa Japonica Group, Hordeum vulgare, Glycine max, Spinacia oleracea.
The sequence of the synthetic peptide used for immunization is 95% homologues with the sequence in AHA4 (AT3G47950), AHA6 (AT2G07560), AHA7 (AT3G60330), AHA8 (AT3G42640), AHA9 (AT1G80660) and AHA11 (AT5G62670); 89% homologues with the sequence in AHA5 (AT2G24520) and AHA10 (AT1G17260).
For more species homologues information, please contact tech support at tech@phytoab.com.

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.