M8370基底胶8.7mg/ml Matrigel
Matrigel(基底胶)
说明:Matrigel是一种细胞外基质的混合物,其中包含laminin和
多种生长因子,用于细胞培养。
浓度:1mg/ml
储存条件:-20℃
BD Matrigel是从富含胞外基质蛋白的EHS小鼠肿瘤中提取出Basement Membrane Matrix基底膜基质胶,其主要成分有Ⅳ型胶原、层粘连蛋白、硫酸肝素糖蛋白、巢蛋白,还包含生长因子和基质金属蛋白酶等。在室温条件下,BD Matrigel聚合形成具有生物学活性的三维基质,模拟体内细胞基底膜的结构、组成、物理特性和功能,有利于体外细胞的培养和分化,可用于对细胞形态、细胞迁移、细胞侵染、生化功能和基因表达等的研究。
BD Matrigel Basement Membrane Matrix基底膜基质胶形成的三维培养基质,可促进肝细胞、上皮细胞、Sertoli细胞、血管内皮细胞、黑色素瘤细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时,Matrigel胶还能影响乳腺上皮细胞的蛋白表达,支持外周神经的新生和牛输卵管上皮细胞的分化。
使用指南:培养细胞时,Matrigel胶可无需稀释直接包被,包被厚度可为0.5 mm(薄层)或1.0 mm(厚层)。BD Matrigel胶也可以在Serum-free Medium无血清培养基中稀释后用于包被培养器皿表面,根据细胞类型和应用目的确定相应Matrigel浓度。欢迎选择Nunc Cell Surveillance Simplified即NUNC CC Inserts插入式细胞培养器,有6孔、12孔、24孔细胞培养板和0.4um孔径、3um孔径、8um孔径等多种规格供您选择购买。
Description 英文介绍
BD Matrigel Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types. These include neurons,5,6 Sertoli cells,7 chick lens,8 and vascular endothelial cells,9 and hepatocytes.10 BD Matrigel will influence gene expression in adult rat hepatocytes11 as well as three dimensional culture in mouse12-15 and human16,17 mammary epithelial cells. It will support in vivoperipheral nerve regeneration,18-20 can be used for metabolism and toxicology studies,21,22 and is the basis for several types of tumor cell invasion assays.23,24 BD Matrigel provides the substrate necessary for the study of angiogenesis both in vitro25,26 and in vivo.27-29 BD Matrigel also supports in vivopropagation of human tumors in immunosupressed mice.30-32
Application
• Invasion Assays
• Metabolism/Toxicology Studies
• Cell Growth and Differentiation
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Quality
• Tested and found negative for bacteria, fungi, and mycoplasma
• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Protein concentrations are determined by Lowry method
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
• BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
Source: Engelbreth-Holm-Swarm Mouse Tumor
Features
• Certified LDEV-Free
• Rigorously validated for functionality
• Extensive quality control exceeding industry standard
• Most extensively references basement membrane matrix
BD Matrigel胶的实验分类应用指南
不同配方的BD Matrigel胶可以满足不同的实验要求,参考如下:
低生长因子(GFR)的Matrigel基底膜基质胶适用于对基质成分要求严格的实验,如细胞信号通路和细胞因子的研究等。
不含酚红的BD Matrigel胶适用于荧光检测或显色反应等分析。
高浓度的BD Matrigel胶适用于研究血管生成、肿瘤细胞迁移和体内肿瘤模型的建立等。
1. BD Matrigel 基底膜基质胶 标准型
BD Matrigel Basement Membrane matrix
5 mL 货号:356234 品牌:BD Biocoat
10 mL 货号:354234 品牌:BD Biocoat
5×10 mL 货号:356235 品牌:BD Biocoat
10 mL 高浓度(HC)货号: 354248 品 牌:BD Biocoat
2. BD Matrigel 基底膜基质胶,无酚红BD Matrigel Matrix Phenol Red-free
10 mL 标准型 货号:356237
10 mL 高浓度(HC)货号:354262
3. BD Matrigel 基底膜基质胶,低生长因子Growth factor reduced (GFR) Matrigel matrix
5 ml 标准型 货号:356230
10 ml 标准型 货号:354230
10 ml 高浓度(HC) 货号:354263
4. BD Matrigel 基底膜基质胶,GFR,低生长因子,无酚红
10 ml 标准型 货号:356231 品 牌:BD Biocoat
BD Biocoat系列产品介绍
BD Biocoat系列产品主要包括BD Biocoat细胞培养器皿,BD Biocoat细胞培养系统,BD Biocoat细胞外基质,BD Biocoat细胞相关检测系统,BD Biocoat细胞因子和BD Biocoat培养添加剂等。这些产品能在BD Falcon多孔系列培养皿,培养瓶,培养玻片和玻璃盖的表面,预包被不同成分的细胞外基质和粘附因子,为细胞提供更接近体内的生长环境,具有方便即用,质量保证,性能可靠,质控良好,种类丰富,用途广泛等特点。
方便即用: 节约体力和实验时间
质量保证:
可以促进多种原代细胞培养的贴壁,生长和分化
可以用于细胞的多种功能研究,包括迁移,趋化,侵袭等
培养基和培养添加剂均通过支原体和内毒素测试
包被成分经过SDS-PAGE纯化
质控良好: ISO9001生产环境严格监控,保证产品批次与批次间的一致性
BD Biocoat品牌的部分产品:
BD Biocoat 356234 Matrigel Basement Membrane Matrix 5mL Store at -20°C. Avoid multiple freeze-thaws.
BD Biocoat 356705 PLATE COLLAGEN I 384WELL BLACK/CLEAR RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356694 PLATE POLY-D-LYSINE 384WELL WHT CLR RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356697 PLATE POLY-D-LYSINE 384WELL BLK CLR RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356693 PLATE POLY-D-LYSINE 96WELL WHT CLR RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C)
Biocoat 356234 Matrigel Basement Membrane Matrix 5mL Store at -20°C.Avoid multiple freeze-thaws.
Biocoat 356691 PLATE POLY-D-LYSINE 96WELL WHITE RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356692 PLATE POLY-D-LYSINE 96WELL BLK CLR RO 1 CAS,Storage at:Cntr. Tmp(4 to 30C) BD Biocoat 356662 PLATE PDL 384WELL CLEAR BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356660 PLATE PDL 384WELL WHITE/CLEAR BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356654 FLASK GEL 75CM2 250ML CNTD PLUG BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356653 DISH GELATIN 100X20MM STYLE BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) BD Biocoat 356652 PLATE GELATIN 6WELL BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) Biocoat 356651 PLATE PDL 96WELL WHITE/CLEAR BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C) BD Biocoat 356640 PLATE PDL 96WELL BLACK/CLEAR BP RT 1 CAS,Storage at:Cntr. Tmp(4 to 30C)
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北京索莱宝科技有限公司(Beijing Solarbio Science & Technology Co.,Ltd. )是一家从事生物学试剂及试剂盒的高科技生物企业。总部位于北京,并在全国设有经销或代理机构。产品因可靠而稳定的质量和完善的售后服务确立了“Solarbio”良好的品牌形象。公司秉承“以客户为中心、以产品为保障、以诚信为基础、以创新为宗旨”的发展理念,拥有专业的研发和质量检测团队以及先进的仓储和物流系统。“Solarbio”已经得到全国科研工作者的认可,成为广大经销商推崇的知名品牌。
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