产品描述: MaxRecovery™ Human Buccal Cell DNA Extraction Kit(人颊粘膜细胞DNA提取试剂盒)是用来从人颊上皮细胞(也叫颊粘膜细胞)中回收高分子量DNA的。该试剂盒包含可以进行非侵入性取样的细胞学刷子及从回收的细胞中提取DNA的试剂。该试剂盒还包含了一对PCR引物,可以扩增单拷贝的X染色体连锁的人类基因,用来验证DNA提取是否成功。如本操作手册最后部分所示,本试剂盒中包含的人颊粘膜细胞DNA 和引物对于解决PCR 反应中出现的问题是非常有用的。
测量/试剂盒: 该试剂盒含可做30 次提取的材料
保质期: 最适条件下储存6个月
储存: 推荐按下表储存试剂盒组分
试剂盒组分:
Kit ContentsAmountStorage
Cytology brushes30 eachRoom temp
Lysis Solution24 mLRoom temp
Neutralization Solution5 mLRoom temp
Human gDNA Primer Set #1*0.5 mL-20°C
*Note: the amplicon produced using these primers is 699 bp. It is derived from the single-copy gene for Factor IX human blood clotting protein (Accn # K02402), which is located on the X-chromosome. The primer binding sites are in the intron flanking exon 8. The primers are provided as a mixture containing 5 uM concentration of each primer, Forward and Reverse.