JMJD2C Homogeneous Assay Kit
Catalog # 50415
Size: 384 reactions

DESCRIPTION: The JMJD2C Homogeneous Assay Kit is designed to measure activity of
the JMJD2C for screening and profiling applications. JMJD2C is a JmjC-domain protein
that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. The JMJD2C
Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated
histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified
JMJD2C for 384 enzyme reactions. The key to the JMJD2C Homogeneous Assay Kit is a
highly specific antibody that recognizes demethylated substrate. With this kit, only three
simple steps on a microtiter plate are required for methyltransferase detection. First, a
sample containing JMJD2C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Note: The kit is also suitable for use with cell extracts, but non-specific demethylase activity may be detected.
 

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
 
CONTRAINDICATIONS:   Green  and blue dyes  that absorb  light  in  the AlphaScreen
signal emission  range  (520-620 nm),  such as Trypan Blue. Avoid  the use of  the potent
singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at more than 1% leads to a
signal  reduction  due  to  the  presence  of  excess  biotin  and  iron  in  this medium. MEM,
which lacks these components, does not affect AlphaScreen assays. 
 
STABILITY:  At least one year from date of receipt when stored as directed.

REFERENCE:  Whetstine JR ea. Cell 2006; 125: 467.