JMJD2E Homogeneous Assay Kit
Catalog # 50417
Size: 384 reactions

DESCRIPTION: The JMJD2E Homogeneous Assay Kit is designed to measure activity of
JMJD2E for screening and profiling applications. JMJD2E, also known as KDM4DL
(lysine (K)-specific demethylase 4D-like) is a JmjC-domain protein that exhibits
demethylation activity toward H3-K9Me3 and H3-K36Me3. The JMJD2E Homogeneous
Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide
substrate, primary antibody, demethylase assay buffer, and purified JMJD2E for 384
enzyme reactions. The key to the JMJD2E Homogeneous Assay Kit is a highly specific
antibody that recognizes demethylated substrate. With this kit, only three simple steps on a
microtiter plate are required for methyltransferase detection. First, a sample containing
JMJD2E enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor
beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

CONTRAINDICATIONS: Green and blue dyes that absorb light in the AlphaScreen
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent
singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at > 1% leads to signal
reduction due to the presence of excess biotin and iron in this medium. MEM, which
lacks these components, does not affect AlphaScreen assays.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCE: Whetstine, J.R. et al., Cell 2006; 125: 467.