PDE4D3 TR-FRET Assay Kit

Catalog # 60701
Size: 96 reactions

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle
contractility, and has been identified as a potential risk predictor for ischemic stroke.
Additionally, PDE4D has been associated with asthma pathophysiology and bone
formation. The PDE4D gene encodes at least 9 different isoforms. The TR-FRET
PDE4D3 Assay Kit is designed for identification of inhibitors of PDE4D3 using TR-FRET
(Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is
based on the binding of phosphate groups from FAM-labeled nucleotide
monophosphates generated by PDE4D3 and from terbium donors to the nanoparticle
binding agent. The binding will result in energy transfer from terbium to FAM and the
fluorescent intensity change can be measured using fluorescence reader.

The TR-FRET PDE4D3 Assay Kit comes in a convenient 96-well format, with purified
PDE4D3 enzyme, fluorescently labeled PDE4D3 substrate (cAMP), binding agent, and
PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a
microtiter plate are required for the PDE4D3 activity assay. First, the fluorescent-labeled
cAMP is incubated with a sample containing PDE4D3 for 1 hour. Second, a binding
agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then,
fluorescence intensity can be measured using a fluorescence reader.

APPLICATIONS: Great for screening small molecular inhibitors for drug discovery and
HTS applications.

COMPONENTS:

REFERENCE(S): Bender. A., et al. Pharmacol. Rev. 2006; 58: 488-520.

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