Homogeneous Caspase-3 Assay Kit
Homogeneous Caspase-3 Assay Kit
Catalog #80700
Size: 96 reactions
DESCRIPTION: Caspase-3 is a member of the caspase (cysteine aspartate protease)
family of proteins, and has been shown to be an executioner protein of apoptosis. The
Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure
Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-
well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity
measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent
Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using
this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3
activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified
Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be
measured using a fluorescence reader.
COMPONENTS:
APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
STABILITY: One year from date of receipt when stored as directed.
REFERENCES:
1. Liu, J., et al., Bioorg. Med. Chem. Lett., 1999 Nov 15;9(22):3231-6.
2. Riedl, S.J., et al., Cell, 2001 Mar 9;104(5):791-800.
Click here to view and download product data sheet (PDF).
Aliases: Caspase 3, Caspase3, CASP3
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