NEK2 (Full length, human, recombinant, N-terminal His tag)
Catalog #: 40009

Size: 10 µg

Description: Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal
HIS6 tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Fomulated in: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween-20, 40% glycerol, and 3
mM DTT.

Stability: >6 months at –80°C

Purity: >70 % by SDS-Page

Specific Activity: 100 U/mg. One unit is defined as the amount of enzyme that will
phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.
Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate,
0.5 mM ATP, 0.3 mg/ml MBPsubstrate, and 0.2 ug/ml NEK2.

Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity
profiling.

References:
1. Fletcher, L. etal., Biochim. Biophys. Acta 1744 (2), 89-92 (2005)
2. Fardilha, M. etal., Ann. N.Y. Acad. Sci. 1030, 468-478 (2004)