Mouse Monoclonal to CD162 / PSGL-1 TC2 (IgG1)

Background

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.

Entrez Gene: 12q24

Specificity

The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34⁺ bone marrow cells.

Regulatory Status

RUO

Immunogen

Human thymocytes

Species Reactivity: Human Negative Species:

Applications: Flow CytometryRecommended dilution:1.5 - 2.5 μg/ml Positive control:human peripheral blood Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references *Moore KL: Structure and function of P-selectin glycoprotein ligand-1. Leuk Lymphoma. 1998 Mar;29(1-2):1-15. [Abstract] *Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ. PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity. 1999 Sep;11(3):369-78. [Abstract] *Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. J Immunol. 2000 Sep 1;165(5):2764-72. [Abstract] [Full Text] *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002). *Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. J Clin Immunol. 2004 Jan;24(1):62-5. [Abstract]