Background

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages.
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.


Entrez Gene: 16p11.2

Specificity

The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.

HLDA VI., WS Code A054

Regulatory Status

RUO

Immunogen

Human granulocytes

Species Reactivity: Human Negative Species:

Applications: Flow CytometryRecommended dilution:1 μg/ml Functional ApplicationThe antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references *Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20. [Abstract] [Full Text] *Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606. [Abstract] *Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98. [Abstract] Product Specific References Leukocyte Typing VI. Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Fig. 1.



Fig. 1. Surface staining of human peripheral blood cells with anti-CD11b (MEM-170) Alexa Fluor® 700.