Fer2 | ferrritin 2
product information
background
Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.
immunogen
KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii
antibody format
rabbit,
polyclonal
serum,
lyophilized
quantity
200 µl
- for reconstitution add 200 µl of sterile water
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications
western blot (WB)
related products
AS06 126 Fer1 | ferritin 1(pre-apoferritin)
Collection of antibodies to Chlamydomonas proteins
additional information
to be added when available
application information
recommended dilution
1 : 1000
expected | apparent MW
32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii
confirmed reactivity
Chlamydomonas reinhardtii
predicted reactivity
Chlamydomonas reinhardtii
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
a picture with a western blot application example can be found in the reference given below
selected references
Long et al. (2008) Genetics 179: 137-147
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