GLUC | beta-glucosidase, Biotin conjugated
product information
background
beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon upon β1->4 bonds linking two glucose or glucose-substituted molecules.
immunogen
native beta- glucosidase purified from almonds
antibody format
rabbit
polyclonal
purified IgG in phosphate buffer saline,
lyophilized
quantity
1 ml
for reconstitution please add 1 ml of sterile destilled water.
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
related products
AS09 449 | anti-beta glucosidase (total IgG)
AS09 451 | anti-beta glucosidase (affinity purified)
additional information
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
application information
recommended dilution
1: 1000 - 1: 100 000 for techniques listed above
expected | apparent MW
n.a.
confirmed reactivity
Prunus dulcis
predicted reactivity
n.a.
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
selected references
to be added when available
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