Description:

Rabbit polyclonal antibody

  Background:

NDHC is an NADH dehydrogenase D3 subunit of the chloroplast NAD(P)H dehydrogenase complex.

  Synonyms:

NDHC, HIGH CHLOROPHYLL FLUORESCENCE 164

  Immunogen:

KLH-conjugated synthetic peptide of NDHC derived from Arabidopsis thaliana ATCG00440.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

14 / 13 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Glycine max, Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Gossypium raimondii, Triticum aestivum, Populus trichocarpa, Zea mays, Setaria viridis, Leymus chinensis, Spinacia oleracea, Vitis vinifera, Hordeum vulgare, Oryza sativa Japonica Group, Sorghum bicolor.
For more species homologues information, please contact tech support at tech@phytoab.com.

1 and 2 are thylakoid membrane protein from WT of Arabidopsis thaliana containing 2 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from an ndh mutant of Arabidopsis thaliana containing 2 μg of chlorophyll.
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.