Fluo-3,AM
Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. Therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Fluo-3/AM itself does not bind Ca2+, but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. A new and potentially very important application of Fluo-3/AM is its use in high throughput drug screening.
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