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Agarose beads Conjugated anti-c-Myc Mouse monoclonal antibody

Catalog number：AT0080       Size：1ml

Specificity： Anti- c-Myc Agarose beads(Affinity gel) is a mouse monoclonal antibody that is covalently attached to agarose beads by hydrazide linkage. The antibody binds c-Myc at the N-terminal, C-terminal and internal locations of fusion proteins.

Tested applications:  Immunoprecipitation (IP),

40 ml of gel suspension (~20μl of packed beads) for ~1000μg of crude total protein solution.

Optimal dilutions/concentrations should be determined by the end user.

Reactivity:  The antibody detects and captures c-Myc -Tagged fusion proteins overexpressed in mammalian cells or E.coli.

Immunogen:  This monoclonal antibody is produced by immunizing mouses with a synthetic peptide (KLH-coupled) containing EQKLISEEDL.

Form:  The productis supplied as a 50% suspension in 50% glycerol with 10 mM sodium phosphate and 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide

Binding capacity:  >10 nmoles of c-Myc-tagged fusion protein per 1 ml of settled gel.

Isotype:  Mouse IgG1

Recommended IP Lysis buffer for mammalian cells: 50 mM Tris HCl, pH 7.4, with 150 mM NaCl, 1 mM EDTA, and 1% TRITON X-100.

Storage instruction:

The gel is supplied in 50% glycerol, so the gel can be stored at –20 °C for maximum stability. The unopened product is stable for one year when stored as indicated.

产品简述：标签抗体偶联的agarose beads又名标签抗体affinity gel，可无需Protein A /G beads，直接抓取目的标签融合蛋白，主要用于对抗体亲和力和特异性要求极高的免疫沉淀实验（IP）、免疫共沉淀（Co-IP）、染色质免疫共沉淀实验（ChIP）。 货号 标签抗体偶联亲和凝胶 Host 应用实验 Reactivity 规格 AT0078 Anti-Flag-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 1ml AT0079 Anti-HA-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 1ml AT0080 Anti-C-Myc-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 1ml AT0084 Anti-His-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml AT0082 Anti-VSV-G-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml AT0083 Anti-GFP (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml AT0081 Anti-V5-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 2ml AT0087 Anti-S-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml AT0088 Anti-T7-Tag (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml AT0086 Anti-RFP (Agarose Conjugated) affinity gel mouse IP,Co-IP all 0.4ml

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