Product Overview

NutriStem® V9 XF medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA. It contains only the essential components required for long-term maintenance of hES and hiPS cells. NutriStem® V9 XF medium shows superior proliferation rates during long-term cultures, while maintaining the pluripotency of the cells.
NutriStem® V9 XF medium allows culture of hPSC in vitronectin pre-coated culture, as well as direct addition of vitronectin to the medium (no need for pre-coating).

Features

Maintains human ES and iPS cell pluripotency at high passage

Superior results using Vitronectin, may be used with Matrigel coated plates

Save time and skip the time consuming precoating step!

Defined, serum-free, xeno-free

Produced under cGMP

Supports long-term expansion

Long weekend-free (up to 60 hours

Cytokine- free basal medium, applicable for EBs formation, reprogramming, and differentiation

The proteins used: HSA (Human Serum Albumin), rh bFGF, rh TGFβ, human transferrin and recombinant human insulin.

Contains Alanyl glutamine. Does not contain antibiotics.

Sample Data

Long-term Expansion

Nucleocounts performed on equal volume of cell suspension Chemometech, (count of aggregated cells) during long-term expansion of H1 cultured in NutriStem® V9 XF medium and E8 under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF. NutriStem® V9 XF medium shows superior proliferation rate in long-term culture (over 20 passages)