细胞计数

Authur荧光细胞计数分析仪

基本信息
产品名称:
Authur荧光细胞计数分析仪
英文名称:
Authur fluorescence cell counting analyzer
国产/进口:
进口
产地/品牌:
韩国/NanoEntek
型号:
Arthur
参考报价:
总点击数:
30115
更新日期:
2025-05-14
产品类别:

性能参数

 

Authur荧光细胞分析仪是是集双荧光通道的显微镜功能、图像分析和细胞计数于一体的仪器。这台自动图像采集分析设备为细胞计数,细胞活率以及细胞凋亡和细胞周期检测提供一体化解决方案 。
 
Authur荧光细胞分析仪特点:
 
• 高精度
• 可以获得精确地,全面的实验结果
• 可以获得细胞图片和细胞计数
 
• 筛选细胞
• 多功能多通道
• 适用广泛的真核细胞
• 也适用于悬浮细胞的各种实验
 
• 快速细胞计数及分析
• 1分钟内3通道的细胞计数
• 只需要25ul样本
 
• 使用简便
• 无需校正,一体机触摸屏操作
 
 
Authur荧光细胞分析仪应用
 
Cell viability assay


Cell line: Jurkat
 

 

 

 

 

 

 

Figure 2. Comparison of GFP expression profile in two platforms


U-2 OS cells were transfected using the Neon™ transfection system (from Life Technologies) and 0.25 µg of the five different GFP constructs and then analyzed on the Arthur™ and competitor’s product F to determine transfection efficiency. The histograms in panels A (Arthur™) and C (competitor’s product F) show the green fluorescence intensities  and counts for the transfected populations. The percentages of GFP-expressing cells detected with the Arthur™ were consistent with the results from the competitor’s product F. ‘Colored circles’ option makes it easy to identify gated cells (Panel B); colored circles represent: cells expressing GFP (green circles), non-GFP-expressing cells (blue circles). The image in panel D shows the GFP expressing cells before trypsinization under the conventional fluorescence microscope.

 

 GFP expression assay

GFP-fusion construct 1   GFP-fusion construct 2  

 

Annexin V apoptosis analysis

Figure 3. Arthur™ allows confirmation of apoptosis assay using the visual display of the samples


Following 18 hour-treatment of Staurosporine, HeLa cells were stained with apoptosis kit and subjected to Arthur™ and competitor’s product F. The apoptosis kit contains an annexin V–Alexa Fluor® 488 conjugate and PI to differentiate live (annexin V–negative/PI-negative), dead (PI-positive), and apoptotic (annexin V–positive/PI-negative) cells. Using Arthur™, 20 % of the cell population were measured as live, 38 % apoptotic, and 42 % dead, confirming that the Arthur™ provides quantitative data comparable to those collected from competitor’s product F.

Cell cycle assay

 

Figure 4. Comparison of cell cycle profiles in two platforms:
Arthur™ and Competitor’s product F


HeLa cells were treated with Nocodazole (microtubule-destabilizer) or Epothilone B
(microtubule-stabilizer) to arrest cells in G2/M phase. Cells were fixed with 70%
ethanol and stained with Propidium Iodide(PI). The histograms show the percentages
of G0/G1 phase, S phase and G2/M phase of cell cycle analysis for drug treated
HeLa cells on the Arthur™ (A) and Competitor’s product F (B).
The cell cycle analysis of Arthur™ was highly consistent with the results obtained
from competitor’s product F.

 

 

Specifications

 Counting time  10 sec. ~ 2 min.
 Cell measurement range  1 × 105 - 1 × 107 cells/mL
 Cell size range  5 - 60 m
 Sample volume  25 µL
 Optics  3 channels (bright field, green fluorescence and red fluorescence)
 Excitation  Green channel LED: 458 ± 20 nm
 Red channel LED: 530 ± 20 nm
 Filters  Green channel: 466/40 EX, 495 LP Di, 525/50 EM
 Red channel: 543/22 EX, 580 LP Di, 585 LP EM
 Camera  1.3 Mega pixels, 4× objective,
 4× or 16× digital zoom
 USB drive  4 GB
 Operating power  100-240 V, 2.5 A, 120 W
 Frequency  50 / 60 Hz
 Dimension  290 mm (W) × 440 mm (D) × 290 mm (H)
 Weight  8.7 kg (19.4 lbs)
 Operating environment  5 - 40 °C, 20 - 95 %

Ordering information

 Cat. No.

 Description

 Contents

 AT1000  Arthur Novel fluorescence cell counter  Main device, Power supply, USB drive,
 ArthurTM Cell analysis slide (50 slides)
 AC0050  ArthurTM Cell analysis slide  50 slides (100 counts)
 AC0500  ArthurTM Cell analysis slide  500 slides (1,000 counts)
 AB0100  ArthurTM Calibration beads  Alignment beads, Green calibration beads,
 Red calibration beads (100 μL/tube)

 




上海吉谦生物科技有限公司
固定电话:021-60296318
联系手机:13671889138(微信同号),陈经理
E-mail:genemod@163.com
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公司简介
上海吉谦生物是一家以国际领先的高新生物医药技术为核心,并快速拓展国内市场的生物医药产品销售和技术外包服务的高新技术企业。公司作为Genolution的中国总代,以独特先进的核酸提取技术、优良的服务和产品质量、丰富和专业的经验以及踏实作风赢得了国内客户的认可。
      公司目前具备完善的分子生物医学科研项目课题实验外包服务、医学科研项目整体实验外包服务平台,能够为广大客户提供医学科研样本检测、合作研究、开发及生产服务, 我们拥有专业的实验室、先进的实验设备和经验丰富的科研技术人员。主要致力于基因组学、转录组学、蛋白质组学、代谢组学、生物化学、病理检测、基因检测等技术在科研中的应用与推广。通过高度细分、的服务平台,为广大客户提供了完善的技术解决方案和服务。协助发表的SCI及核心期刊上的学术文章,涉及临床医学、肿瘤生物学、免疫学、细胞生物学、分子生物学等生命科学、医学等诸多领域。
       公司2021年成立于上海宝山城市工业园区。 公司拥有标准化的PCR实验室和一流的实验设备和仪器,实验室总面积近500平方米。公司团队有经验丰富的员工近百人,其中生物医学类博士以上学历2人,硕士学历12人。
公司的宗旨是为客户提供一流的产品和服务。我们相信通过不断引进新的产品,以及全体员工的不断努力,新老客户的大力支持,吉谦生物的明天会更好。

售后服务
1.1年无条件保修;终身维护。 2.接收到服务要求后,如电话不能解决问题,36小时内提供现场服务。
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详细地址:
上海市宝山工业园园丰路69号一号楼802室
qq:
2646245(微信同号)
联系电话:

021-60296318

Email:

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