3D上皮细胞培养基
基本信息
产品名称：
3D上皮细胞培养基
英文名称：
CnT-Prime 3D Barrier Culture Medium
国产/进口：
进口
产地/品牌：
瑞士 Cellntec
型号：
500 mL (frozen)
参考报价：
销售商：
总点击数：
1676
更新日期：
2025-05-14
产品类别：
性能参数
CnT-Prime 3D Barrier是一种完全确定的培养基，用于在3D气举培养中优化初级人类表皮角质形成细胞的分化。它完全没有动物或人为成分。CnT-Prime 3D Barrier包含了各种氨基酸、矿物质、维生素和有机化合物，以及经过修饰的生长因子，可以增强屏障功能。它还含有额外的脂质，这可能需要加热到室温才能在解冻后重新溶解(见下文)。它不含苯酚红或抗生素/抗真菌药。
物种
主要用于人类表皮角质形成细胞。也可用于其他物种，包括老鼠。
组织类型
皮肤
规格
500毫升瓶，充分补充生长因子。不需要进一步增加。
产品使用
用于建立具有较高屏障功能的原始人类表皮角质形成细胞的完全分层培养。有关描述3D模型开发的详细说明，请参阅参考资料部分的differentiation protocol。
明确的 :是的
培养条件
这种介质是为在5%的二氧化碳大气中使用而设计的。有关详细的差异化协议，请访问的参考资料部分
质量控制
通过渗透压、pH值和各种离子浓度测试介质的组成。在3D气举培养中，通过区分人的初级上皮细胞来测试介质功能。无细菌，真菌和支原体污染。
装运条件:冷冻装运。
预期用途:只供研究用途。不用于治疗或诊断。
CnT-Prime 3D Barrier Medium
CnT-Prime 3D Barrier is a fully defined medium formulation designed for optimal differentiation of primary human epidermal keratinocytes in 3D airlift cultures. It is completely free of animal or human-derived components. CnT-Prime 3D Barrier contains a full range of amino acids, minerals, vitamins and organic compounds, and modified growth factors to deliver increased barrier function. It also contains additional lipids, which may require warming to room temperature to re-solubilize after thawing (see below). It does not contain phenol-red, or antibiotics / antimycotics.
SPECIES
Developed for primary human epidermal keratinocytes. May be used for other species as well, including mouse.
TISSUE TYPE
Epithelia
PACK SIZE
500 mL bottle, fully supplemented with growth factors. No further additions required.
PRODUCT USE
Developed for the establishment of fully stratified cultures of primary human epidermal keratinocytes with increased barrier function. For a detailed instructions describing the development of the 3D models, please see the differentiation protocols in the resources section of .
MEDIA TYPE
3D-Diff
DEFINED
Yes
ACF
Yes
CULTURE CONDITION
This medium is designed for use in a 5% CO2 atmosphere. For a detailed differentiation protocol, please visit the resources section of
NOTE
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. This is particularly important during differentiation.
STORAGE / SHELF LIFE
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. Once thawed, lipids that have not fully re-solubilised in the cold aqueous medium may be visible as a thin milky layer at the bottom of the bottle. To solubilize these lipids, warm medium to room temperature and swirl gently. Do not thaw medium in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
QUALITY CONTROL
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by differentiating primary human epithelial cells in 3D airlift culture. Free of bacteria, fungi and mycoplasma contamination.
SHIPPING CONDITION
Medium is shipped frozen.
INTENDED USE
For research use only. Not for use in therapy or diagnostics.
LAST UPDATE
2018-04-03
物种
主要用于人类表皮角质形成细胞。也可用于其他物种，包括老鼠。
组织类型
皮肤
规格
500毫升瓶，充分补充生长因子。不需要进一步增加。
产品使用
用于建立具有较高屏障功能的原始人类表皮角质形成细胞的完全分层培养。有关描述3D模型开发的详细说明，请参阅参考资料部分的differentiation protocol。
明确的 :是的
培养条件
这种介质是为在5%的二氧化碳大气中使用而设计的。有关详细的差异化协议，请访问的参考资料部分
质量控制
通过渗透压、pH值和各种离子浓度测试介质的组成。在3D气举培养中，通过区分人的初级上皮细胞来测试介质功能。无细菌，真菌和支原体污染。
装运条件:冷冻装运。
预期用途:只供研究用途。不用于治疗或诊断。
CnT-Prime 3D Barrier Medium
CnT-Prime 3D Barrier is a fully defined medium formulation designed for optimal differentiation of primary human epidermal keratinocytes in 3D airlift cultures. It is completely free of animal or human-derived components. CnT-Prime 3D Barrier contains a full range of amino acids, minerals, vitamins and organic compounds, and modified growth factors to deliver increased barrier function. It also contains additional lipids, which may require warming to room temperature to re-solubilize after thawing (see below). It does not contain phenol-red, or antibiotics / antimycotics.
SPECIES
Developed for primary human epidermal keratinocytes. May be used for other species as well, including mouse.
TISSUE TYPE
Epithelia
PACK SIZE
500 mL bottle, fully supplemented with growth factors. No further additions required.
PRODUCT USE
Developed for the establishment of fully stratified cultures of primary human epidermal keratinocytes with increased barrier function. For a detailed instructions describing the development of the 3D models, please see the differentiation protocols in the resources section of .
MEDIA TYPE
3D-Diff
DEFINED
Yes
ACF
Yes
CULTURE CONDITION
This medium is designed for use in a 5% CO2 atmosphere. For a detailed differentiation protocol, please visit the resources section of
NOTE
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. This is particularly important during differentiation.
STORAGE / SHELF LIFE
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. Once thawed, lipids that have not fully re-solubilised in the cold aqueous medium may be visible as a thin milky layer at the bottom of the bottle. To solubilize these lipids, warm medium to room temperature and swirl gently. Do not thaw medium in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
QUALITY CONTROL
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by differentiating primary human epithelial cells in 3D airlift culture. Free of bacteria, fungi and mycoplasma contamination.
SHIPPING CONDITION
Medium is shipped frozen.
INTENDED USE
For research use only. Not for use in therapy or diagnostics.
LAST UPDATE
2018-04-03
公司简介
普瑞麦迪（北京）实验室技术有限公司坐落于中关村科技园丰台园区，是致力于生命科学实验室整体解决方案的国家高新技术企业。公司秉承“持续创新、以人为本、服务至上、合作共赢”的理念，以“引进全球创新技术、创新产品、助力中国生命科学飞速发展”为使命；聚集了一批行业内的专业精英，包括杰出的留学归国研发和管理人员及国内优秀人才等一起构成公司的精英团队。公司集自主研发和引进国际顶尖技术产品为一体，主营产品涵盖生命科学、医学、环境生物学及化学等领域的高端仪器及试剂。
售后服务
普瑞麦迪公司是专业的流式细胞仪代理和服务商，产品涵盖细胞成像系统、流式细胞仪、分子生物学产品、常规实验室设备及试剂等众多产品。公司有完善的售前、售后、实验室技术团队，有充足的仪器配件、DEMO样机及优秀的技术人员,“服务为本”是普瑞麦迪公司一贯的工作理念。
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