中性蛋白酶
基本信息
产品名称：
中性蛋白酶
英文名称：
Dispase II, Neutral Protease
国产/进口：
进口
产地/品牌：
瑞士 Cellntec
型号：
5*0.1g（干粉）
参考报价：
销售商：
总点击数：
2132
更新日期：
2025-05-14
产品类别：
性能参数
Dispase®是一个中性蛋白酶,劈开纤连蛋白,胶原IV,和一定程度上的胶原蛋白I,可用于单独的上皮组织免受潜在的间质。
组织类型
酶
包装规格
5 x 100毫克(约)粉末(分开管)
产品使用
将4ml PBS直接加入所提供的试管中，重新组合成单个粉末，形成10x溶液。轻轻旋流，确保完全溶解。将10倍的无菌溶液用注射器顶部的过滤器过滤到50毫升的试管中，然后加入36毫升无菌PBS，组成1倍的溶液。如果协议要求在PBS中对组织样本进行一夜培养，则建议在培养基中重新构建粉体，以便随后使用，而不是PBS。根据不同批次的活性，每个批号中所含的粉末数量略有不同。加入的粉末在溶解后，其最终1x活性为2.4 U/mL。这个解决方案可能是储存在4°C 2周内使用,或能整除的为15毫升管和冻结在-20°C在6个月内使用。有关推荐的隔离协议，请访问的参考资料部分
储存/保质期
商店在4°C粉末形式。有效期见标签。应该能整除与PBS调整后,CnT-DNP-10变成所需的数量(例如15毫升),和aliquots refrozen并存储在-20°C在6个月内使用。避免额外的冻融循环。在4°C解冻整除,直到使用存储在这个温度。解冻后2周内使用。
质量控制
无菌和成功分离原代上皮细胞。
运输条件
CnT-DNP-10可以单独在常温下运输，也可以与其他冷冻产品一起运输。
预期用途
只供研究用途。不用于治疗或诊断。
活性浓度
最终的1x溶液的活性约为2.4 U / mL
Dispase II, Neutral Protease, 5 x 0.1 gram
Dispase® is a neutral protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I, and can be used to separate epithelial tissues from underlying mesenchyma.
TISSUE TYPE
Enzymes
PACK SIZE
5 x 100 mg (approx) powder aliquots (separate tubes)
PRODUCT USE
Reconstitute a single powder aliquot by adding 4 mL of PBS directly to the tube supplied, to make up a 10x solution. Gently swirl to ensure complete solubilization. Sterile filter this 10x solution with a syringe-top filter into a 50 mL tube, then add 36 mL of sterile PBS to make up the 1x solution. Where the protocol calls for an overnight incubation of the tissue sample in PBS, it is recommended to reconstitute the powder aliquots in the culture medium to be used subsequently instead of PBS. The quantity of powder included in each aliquot will vary slightly from lot-to-lot, according to the lot-specific activity. The amount of powder included will always deliver a final 1x activity of 2.4 U/mL after solubilisation. This solution may be stored at 4°C for use within 2 weeks, or aliquoted into 15 mL tubes and frozen at -20°C for use within 6 months. For recommended isolation protocols, please visit the resources section of
DEFINED
No
ACF
No
STORAGE / SHELF LIFE
Store at 4°C in powder form. See label for expiry date. After reconstitution with PBS, CnT-DNP-10 should be aliquoted into desired quantities (e.g. 15 mL), and the aliquots refrozen and stored at -20°C for use within 6 months. Avoid additional freeze and thaw cycles. Thaw aliquots at at 4°C, and store at this temperature until use. Use within 2 weeks of thawing.
QUALITY CONTROL
Tested for sterility and successful isolation of primary epithelial cells.
SHIPPING CONDITION
CnT-DNP-10 can be shipped alone at ambient temperature, or included with other frozen products.
COMMENTS
Inhibitors: EDTA; EGTA; Hg2+; heavy metals; not inhibited by serum Activators: Ca2+; Mg2+; Mn2+; Fe2+; Fe3+; Al3+ pH optimum: 6 to 8.5
INTENDED USE
For research use only. Not for use in therapy or diagnostics.
ACTIVITY CONCENTRATION
The final 1 x solution has an activity of approximately 2.4 U / mL
组织类型
酶
包装规格
5 x 100毫克(约)粉末(分开管)
产品使用
将4ml PBS直接加入所提供的试管中，重新组合成单个粉末，形成10x溶液。轻轻旋流，确保完全溶解。将10倍的无菌溶液用注射器顶部的过滤器过滤到50毫升的试管中，然后加入36毫升无菌PBS，组成1倍的溶液。如果协议要求在PBS中对组织样本进行一夜培养，则建议在培养基中重新构建粉体，以便随后使用，而不是PBS。根据不同批次的活性，每个批号中所含的粉末数量略有不同。加入的粉末在溶解后，其最终1x活性为2.4 U/mL。这个解决方案可能是储存在4°C 2周内使用,或能整除的为15毫升管和冻结在-20°C在6个月内使用。有关推荐的隔离协议，请访问的参考资料部分
储存/保质期
商店在4°C粉末形式。有效期见标签。应该能整除与PBS调整后,CnT-DNP-10变成所需的数量(例如15毫升),和aliquots refrozen并存储在-20°C在6个月内使用。避免额外的冻融循环。在4°C解冻整除,直到使用存储在这个温度。解冻后2周内使用。
质量控制
无菌和成功分离原代上皮细胞。
运输条件
CnT-DNP-10可以单独在常温下运输，也可以与其他冷冻产品一起运输。
预期用途
只供研究用途。不用于治疗或诊断。
活性浓度
最终的1x溶液的活性约为2.4 U / mL
Dispase II, Neutral Protease, 5 x 0.1 gram
Dispase® is a neutral protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I, and can be used to separate epithelial tissues from underlying mesenchyma.
TISSUE TYPE
Enzymes
PACK SIZE
5 x 100 mg (approx) powder aliquots (separate tubes)
PRODUCT USE
Reconstitute a single powder aliquot by adding 4 mL of PBS directly to the tube supplied, to make up a 10x solution. Gently swirl to ensure complete solubilization. Sterile filter this 10x solution with a syringe-top filter into a 50 mL tube, then add 36 mL of sterile PBS to make up the 1x solution. Where the protocol calls for an overnight incubation of the tissue sample in PBS, it is recommended to reconstitute the powder aliquots in the culture medium to be used subsequently instead of PBS. The quantity of powder included in each aliquot will vary slightly from lot-to-lot, according to the lot-specific activity. The amount of powder included will always deliver a final 1x activity of 2.4 U/mL after solubilisation. This solution may be stored at 4°C for use within 2 weeks, or aliquoted into 15 mL tubes and frozen at -20°C for use within 6 months. For recommended isolation protocols, please visit the resources section of
DEFINED
No
ACF
No
STORAGE / SHELF LIFE
Store at 4°C in powder form. See label for expiry date. After reconstitution with PBS, CnT-DNP-10 should be aliquoted into desired quantities (e.g. 15 mL), and the aliquots refrozen and stored at -20°C for use within 6 months. Avoid additional freeze and thaw cycles. Thaw aliquots at at 4°C, and store at this temperature until use. Use within 2 weeks of thawing.
QUALITY CONTROL
Tested for sterility and successful isolation of primary epithelial cells.
SHIPPING CONDITION
CnT-DNP-10 can be shipped alone at ambient temperature, or included with other frozen products.
COMMENTS
Inhibitors: EDTA; EGTA; Hg2+; heavy metals; not inhibited by serum Activators: Ca2+; Mg2+; Mn2+; Fe2+; Fe3+; Al3+ pH optimum: 6 to 8.5
INTENDED USE
For research use only. Not for use in therapy or diagnostics.
ACTIVITY CONCENTRATION
The final 1 x solution has an activity of approximately 2.4 U / mL
公司简介
普瑞麦迪（北京）实验室技术有限公司坐落于中关村科技园丰台园区，是致力于生命科学实验室整体解决方案的国家高新技术企业。公司秉承“持续创新、以人为本、服务至上、合作共赢”的理念，以“引进全球创新技术、创新产品、助力中国生命科学飞速发展”为使命；聚集了一批行业内的专业精英，包括杰出的留学归国研发和管理人员及国内优秀人才等一起构成公司的精英团队。公司集自主研发和引进国际顶尖技术产品为一体，主营产品涵盖生命科学、医学、环境生物学及化学等领域的高端仪器及试剂。
售后服务
普瑞麦迪公司是专业的流式细胞仪代理和服务商，产品涵盖细胞成像系统、流式细胞仪、分子生物学产品、常规实验室设备及试剂等众多产品。公司有完善的售前、售后、实验室技术团队，有充足的仪器配件、DEMO样机及优秀的技术人员,“服务为本”是普瑞麦迪公司一贯的工作理念。
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