CD99 兔多克隆抗体--性能参数，报价/价格，图片

基本信息

产品名称：

CD99 兔多克隆抗体

英文名称：

CD99

国产/进口：

国产

产地/品牌：

华安生物/HUABIO

型号：

兔多克隆抗体

参考报价：

2500/100ul

销售商：

杭州华安生物技术有限公司

总点击数：

86

更新日期：

2021-04-19

产品类别：

抗体

性能参数

Fig1: Western blot analysis of CD99 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate

Fig2: ICC staining CD99 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ER1803-81 at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig3: ICC staining CD99 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ER1803-81 at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-81 at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.

Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-81 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-81 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-81 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.

Fig8: Flow cytometric analysis of CD99 was done on 293T cells. The cells were fixed, permeabilized and stained with CD99 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

公司简介

杭州华安生物技术有限公司成立于2007年3月，总部位于中国杭州，是一家从事抗体研发与生产的国家高新技术企业。公司致力于为全球的科研工作者和工业客户提供高品质的抗体试剂及抗体服务。
公司的产品线包括兔单抗、小鼠单抗、兔多抗、二抗、蛋白/多肽、基因敲除细胞系、抗体检测用相关试剂等。2015年，公司开始专注于高通量重组兔单克隆抗体的研发与产业化生产。
同时，公司也为客户提供定制抗体的开发、纯化和筛选服务。迄今为止，公司已累计完成超过 3000个定制抗体项目，涉及植物、动物、医学、水产、病毒等不同的科研领域。用户使用公司抗体在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等杂志上发表文章。
公司不但专注于自身技术体系的完善和升级，还注重推动产学研合作：与浙江大学、四川大学、西南大学、中国科学院遗传与发育生物学研究所等知名院校共同承担省级和国家级课题，与杭州精准医药研究中心进行深度合作，进行技术成果交流和共享。

Hangzhou HuaAn Biotechnology Co., Ltd (HuaBio) was founded in 2007. Our headquarter is located in Hangzhou, China. With over 3,000 antibodies developed, HuaBio is committed to providing high-quality antibodies and antibody services to our customers. We have products including recombinant rabbit monoclonal antibodies, mouse monoclonal antibodies, rabbit polyclonal antibodies, phosphor-specific antibodies, secondary antibodies.

HuaBio is also committed to providing the highest quality in customized antibody services . By now, we have delivered more than 3000 customized antibodies for specific applications in various fields (e.g.Neuroscience, Epigenetics, Stem Cells, Microbiology, Signal Transduction Apoptosis and etc. )

售后服务

如有售后问题可及时联系我们，我们会有售后人员为您解决问题，联系电话0571-89986345。