描述
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With |
Without |
• High Glucose |
• HEPES |
• L-glutamine |
|
• Phenol Red |
|
• Sodium Pyruvate |
|
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO
2environment to maintain physiological pH.
Product use
For human
ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File(DMF).
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
规格
Glutamine: |
L-Glutamine |
Phenol Red Indicator: |
Phenol Red |
Form: |
Liquid |
Glucose: |
High Glucose |
Serum Supplementation: |
Standard Serum Supplementation |
HEPES Buffer: |
No HEPES |
Sodium Pyruvate Additive: |
Sodium Pyruvate |
Product Line: |
Gibco® |
Product Size: |
500 mL |
Volume (Metric): |
500 ml |
Green Features: |
Sustainable packaging |
Shipping Condition: |
Room Temperature |
其它规格:
品牌 |
货号 |
品名 |
规格 |
Fermentas |
K1622 |
REVERTAID 1ST CDNA SYNTH KIT |
1盒 |
Fermentas |
26617 |
PAGERULER PRESTAINED PROTEIN |
10*250ul |
Invitrogen |
11668-027 |
脂质体2000[Invitrogen 11668-027] |
0.75ml |
Invitrogen |
11668-019 |
脂质体2000[Invitrogen 11668-019] |
1.5ml |
Invitrogen |
L3000-008 |
转染试剂lipo3000 LIPOFECTAMINE 3000 |
0.75ml |
Invitrogen |
L3000-015 |
转染试剂lipo3000 LIPOFECTAMINE 3000 |
1.5ml |
Invitrogen |
15596-026 |
一步法总RNA提取试剂 TRIzol Reagent[Invitrogen 15596-026] |
100ml |
Invitrogen |
15596-018 |
一步法总RNA提取试剂 TRIzol Reagent[Invitrogen 15596-018] |
200ml |
Invitrogen |
C11875500BT |
国产培养基RPMI 1640,无HEPES、丙酮酸钠 |
500ml |
Invitrogen |
C11995500BT |
国产培养基DMEM高糖,无HEPES |
500ml |
Invitrogen |
25200-056 |
0.25%胰蛋白酶溶液 Trypsin-EDTA (0.25%)[Invitrogen 25200-056] |
100ml |
Invitrogen |
25200-072 |
0.25%胰蛋白酶溶液 Trypsin-EDTA (0.25%)[Invitrogen 25200-072] |
500ml |
Invitrogen |
12800-017 |
DMEM高糖培养基粉末 含丙酮酸钠 [Invitrogen 12800-017] |
10*1L |
Invitrogen |
12100-046 |
DMEM高糖培养基粉末[Invitrogen 12100-046] |
10*1L |
Invitrogen |
31600-034 |
DEME低糖培养基[Invitrogen 31600-034] |
10*1L |
Invitrogen |
31800-022 |
1640培养基[Invitrogen 31800-022 10*1L] |
10*1L |
Invitrogen |
18064-071 |
18064-071 SuperScript II反转录酶 |
4*10000u |
Invitrogen |
14175-095 |
HBSS[Invitrogen 14175-095] |
500ml |
Invitrogen |
17504-044 |
B-27无血清添加剂 [Invitrogen 17504-044] |
10ml |
Invitrogen |
17502-048 |
N-2 添加剂 [Invitrogen 17502-048] |
5ML |
Invitrogen |
21103-049 |
Neurobasal神经细胞培养基{Invitrogen 21103-049} |
500ml |
Invitrogen |
10888-022 |
Neurobasal-A神经细胞培养基 [Invitrogen 10888-022] |
500ml |
Invitrogen |
R25001 |
博莱霉素[Invitrogen R25001] |
1.25ml |
Invitrogen |
13778-150 |
Lipofectamine RNAiMAX Transfection Reagent转染试剂 [Invitrogen 13778-150] |
1.5ml |
Invitrogen |
15140-122 |
青链霉素双抗 (10,000 U/mL)[Invitrogen 15140-122] |
100ml |
Invitrogen |
31985-070 |
Opti-MEM I减血清培养基 [Invitrogen 31985-070] |
500ml |
Invitrogen |
25030-081 |
L-谷氨酰胺100倍浓缩液 [Invitrogen 25030-081] |
100ml |
Invitrogen |
15338-100 |
15338-100 脂质体LTX,含Plus试剂 |
1ml |
Invitrogen |
18064-014 |
18064-014 SuperScript II反转录酶 |
10000u |
更多
原位杂交仪查看www.lingyi17.cn