AfeI内切酶
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| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(BamHI-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | AGC↑GCT |
| 来源 | E.coli strain that carries the cloned Afe I gene from Alcaligenes faecalis T2774 |
| Assayed on | Lambda DNA(BamHI-digest) |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 80%of DNA pBR322 fragments can be ligated and recut. |
| Non-specific hydrolisis | fragments can be ligated and recut.|Non-specific hydrolisis No nonspecific activity was detected after incubation of 1μgof DNA with 40 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | Aor51HI,Eco47III,FunI |
| 货号 | 名称 | 规格 |
| BTN51206 | Taq DNA聚合酶 | 500U |
| BTN120309 | T3 RNA聚合酶 | 1000U |
| WE0236 | 大片段Bst DNA聚合酶 | 800U|4000U |
| SV0108 | BccI限制性内切酶 | 5KU|1KU |
| SV0292 | CspCI限制性内切酶 | 2500U|500U |
| SV0572 | NsiI-HF限制性内切酶 | 5KU|1KU|500U |
| SV0593 | PluTI限制性内切酶 | 2500U|500U |
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