特别提示：包括四环素盐酸盐溶液(50mg/ml)在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：四环素盐酸盐溶液(50mg/ml)
英文名称：Tetracycline HCl Solution
产品货号：RFT185
产品规格：5×1ml

四环素类抗生素主要是与细菌核糖体30S亚单位的A位特异性结合，阻止tRNA在该位置上的联结，阻止肽链延伸，从而抑制细菌蛋白质合成。四环素类还可引起细胞膜通透性改变，使得重要成分外漏，从而抑制细菌生长繁殖。四环素盐酸盐的水溶性好，生物利用度比四环素碱好，故常用于临床及研究领域。四环素盐酸盐溶液由四环素盐酸盐溶于水组成，经0.22μm过滤除菌，可以直接添加到培养基中。一般工作浓度为10～50 μg/ml，其中严紧型质粒常采用10 μg/ml，松弛型质粒常采用50 μg/ml。

使用方法：
根据实验具体要求操作，一般四环素在LB中终浓度为10～50μg/ml。可以大体按照1/1000 LB体积加入50mg/ml Amp溶液，如100ml LB中加入100μl 四环素 50mg/ml。

注意事项：
1、尽注意无菌操作，避免污染。
2、避免反复冻融。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、镁离子是四环素的拮抗剂，四环素抗性菌的筛选应使用不含镁盐的培养基(如LB培养基)。

储存条件：-20℃，有效期6个月。

根据您的关注的四环素盐酸盐溶液(50mg/ml)试剂，您可能还对以下产品有需求：



名称：诺氟沙星
货号：QN0053
规格：5g
CAS：70458-96-7
分子式：C16H18FN3O3
分子量：319.33
储存条件：2～8℃
别名：氟哌酸
性状：类白色至淡黄色结晶性粉末，无臭，味微苦。暴露在空气中易吸湿，形成半水合物。遇光色渐变深。

名称：
货号：QN0087
规格：2mg|10mg
本品常用作细胞分裂抑制剂、DNA合成抑制剂、噬菌体诱导剂。

CAS：50-07-7
分子式：C15H18N4O5
分子量：334.3
储存条件：2～8℃
纯度：98%
性状：蓝紫色有光泽的结晶
别名：自力霉素C
溶解性：溶于水参考浓度0.5mg/ml。

产品说明：
Mitomycin C is produced by a strain of actinomyces named Streptomyces caespitosus. The structural formula of Mitomycin C is a combination of three of the representative anticancerous radicals, quinone, urethane and aziridine group. It is supplied in a vial containing 2 mg Mitomycin C，The other In-active ingredient in Mitomycin-C is Sodium Chloride.
Mitomycin C is widely used among the Mitomycin group of antibiotics because it is the least toxic and with the most potent antitumor activity. It has extraordinarily strong activity against Ehrlich ascites tumor cell.It also possesses strong bactericidal action against gram-positive and gram-negative organisms.
Mitomycin C inhibits DNA synthesis. It reacts covalently with DNA, in vivo and in vitro, forming cross-links between the complementary strands of DNA. This interaction prevents the separation of the complementary DNA strand, thus inhibiting DNA replication. Mitomycin C is one of the few antibiotics known to interact with DNA in this fashion.
The recommended concentration for eukaryote cell culture use is 10-50 µg/mL.
Preparation Instructions
Mitomycin C is soluble in water.The solubility at 0.5 mg/mL (1 vial/4 mL) in water yields a clear blue solution. The contents of one vial dissolved in 4 mL water produces a solution that is 1.5mM Mitomycin C. A 0.5% solution in water has a pH of 6.0-7.5.A saturated solution has a pH of 5.0-9.0.
Mitomycin C undergoes rapid degradation in acidic solutions (pH less than 6.0). It is most stable in aqueous solutions at pH 6-9. Generally, solutions at pH 6-9 can be stored at 0-5℃ for up to a week. If a precipitate forms, a fresh solution should be prepared. The precipitated solution has proven to be toxic to cells. Stock solutions should also be stored in the dark since it is readily decomposed by light.
A study has shown that the amount of Mitomycin C in a culture medium (at 38℃) containing antibiotics with fetal calf serum was reduced by 29% after 30 minutes and by 53% after 60 minutes.

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