Lonza AMAXA Lonza Nucleofector 2B /II 细胞核转染仪

单孔细胞核转染仪

Ÿ   优化的电穿孔技术

Ÿ   独特的细胞特异性电转染程序

Ÿ   程序的不断更新升级

Lonza(原Amaxa)公司的Nucleofection®技术在传统的电穿孔技术基础上取得了突破性进展，通过调整优化电转参数（内存转染程序），结合细胞特异性的转染试剂直接把外源基因导入原代细胞和传代细胞的细胞浆和细胞核中。在保证高效转染的同时，也提高了细胞的存活率；

世界上唯一直接把外源基因导入细胞核的创新型技术！

世界上唯一能高效转染原代细胞、悬浮细胞的非病毒转染技术！

世界上唯一能在数小时内完成从实验操作到结果分析全过程的转染技术！

全球共享细胞优化转染程序数据库；

高效转染DNA、RNA、peptide等各种底物；

已发表超过5000篇文献（www.lonza.com/nucleofection-citations ）。

全球公认的最高效转染技术，它综合应用传统的电穿孔技术及细胞特异性的细胞核转染液，通过调整优化电转参数（内存程序），直接把外源基因导入原代细胞和传代细胞核中。针对免疫细胞、神经细胞、干细胞等几乎所有难转染的细胞系或原代细胞或悬浮细胞。
 

Transfection is an essential tool for numerous applications, including studies of gene expression. However, transfection of cells with common methods such as calcium phosphate, DEAE-dextran, liposome-mediated transfer, electroporation or viral vectors is limited because of low transfection efficiency and the extended time required for analysis of gene expression. Amaxa Biosystems has a new electroporation-based transfection method that enables the DNA to directly enter the nucleus while cells are stabilized in an electric field by treatment with a specific solution. The method is called “Nucleofection” and the electoporator system is called Nucleofector II. The system has built-in optimized programs for most common cell types.

The transfection procedure is simple and straightforward with these basic steps: (1) Preparing cells to be transfected with the nucleofection solution appropriate for the cell type, (2) Adding the nucleic acid to the prepared cells and transferring the mixture to a cuvette (provided in the kit), (3) Placing the cuvette in the system, electroporating cells with the optimized program for the cell type, (4) Harvesting cells from the cuvette and dispersing on to media contained in a dish. The whole process can be done in less than 30 minutes.

The good part about the system is that the method is easy, simple (some optimization required with regard to the appropriate amount of DNA and the type of program used for each cell type), sensitive, requires minimal expertise and no prior plating of cells to be transfected is needed. This in contrast to the alternative methods which are limited by the need to plate cells at a particular density 24-48 hours prior to transfection. In addition, these methods are labor and time intensive. Amaxa has nucleofection kits with optimized protocols available for most common cell types. As nucleic acids are delivered straight into the nucleus, nucleofected cells can be ready for analysis after 2–6 hrs of transfection.

 

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