The PSP Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Transportation of the stabilized DNA can be carried out in the DNA Stabilizer solution without refrigeration at ambient temperature. The purification kit is designed for DNA isolation from pathogenic microorganisms and from the host organism.

• All-in-one product - from collection to isolation: pre-filled collection tubes with Stool DNA stabilizer
• Samples are stable at room temperature for 3 months for easy transport and storage
• Inactivation of DNases and preservation of the microorganism titre
• For In Vitro Diagnostic Use (CE-IVD)*

For the DNA extraction process a small aliquot of the total volume is used, and the residual sample can be stored in the DNA stabilization buffer at -20°C for further extractions. The PSP Spin Stool DNA Plus Kit uses a precleaning step, optimized buffer and essential washing conditions to remove potent inhibitors very efficiently. The purified DNA is ready to use for subsequent downstream applications:

• PCR applications
• hybridization
• genetic typing
• pathogen typing
• mutation analysis
• sequencing
• ancestry research
• feed and food determination


^{*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.}

Total DNA was isolated using the PSP Spin Stool DNA Plus Kit and a competitor kit Q from 20 different stool samples according to the standard protocols. The total DNA concentration was measured on a NanoDrop (figure A) and the respective microorganism DNA content (from Bifidobacterium sp., Escherichia coli, Faecalibacterium prausnitzii, Lactobacillus sp.) was analyzed and measured via quantitative real-time PCR (figure B).

Data kindly provided by S.C. Bischoff, University Hohenheim, Stuttgart, Germany
 

Amplification of different target sequences of extracted bacterial DNA (H. pylori) using the PSP Spin Stool DNA Plus Kit from human stool samples stored at room temperature (without refrigeration) for 3 days with Stool Stabilizer in a Stool Collection Tube.