RAS-related protein Rab10 (RAB10) belongs to the RAS superfamily of small GTPases. RAB10 is a key regulator of intracellular membrane trafficking by orchestrating the biogenesis, transport, tethering, and fusion of membrane-bound organelles and vesicles. LRRK2-mediated phosphorylation may cause deficits in endolysosomal trafficking pathways modulated by RAB10, contributing to neurodegenerative disease onset. RAB10 is associated with Alzheimer’s disease and Parkinson’s disease.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-RAB10 (Thr73) assay is a sandwich immunoassay for the quantitative detection of phospho-RAB10 (Thr73) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.

• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.

• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.

• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates

• Antibody modulators

• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays

• Receptor activation studies

• Screening