pl级/nl级/非接触式微量移液/分液系统

 选配压电式的皮升分液模块（SiJet）、纳升分液模块（PipeJet），进行皮升、纳升级别的液体处理

 标配成像质控相机TopView Camera模块，实现目标区域的定位质控


技术原理




产品特点



应用方向

• 芯片研发生产
• 药物筛选
• 合成生物学
• 微流控芯片点样
• 针头药物包被
• 材料测试(燃料电池等)
• PCR反应体系setup
• 质谱样品制备

典型案例

应用案例1 精准分液
利用TopView模块，BioSpot工作站能够精确定位点样/分液位置，实现点样前位置确定和点样后位置质控



图1 TopView模块定位点样/分液位置

 
应用案例2 针头药物包被
BioSpot工作站可以精准将液体包被在目标微针处（图2）



图2 BioSpot工作站识别并定位微针位置（左），然后将液体点印在微针处（中、右）

 
应用案例3 侧向层析试纸生产
研究背景

利用BIOSPOT工作站开发一款基于等位基因特异性的多重侧向流检测（LFA）试纸条，用于Kidd、Duffy、MNS 三大血型系统的 7 个关键等位基因分型（图3）。



图3 通过 BIOSPOT工作站以 50 纳升体积点样。每个 2×5 阵列包含 10 个液滴（8 个寡核苷酸探针 [1-8] 和 2 个 BSA 对照 [9]）。



图4：LFA 基因分型检测流程示意图：(1) 侧向流检测条结构示意图；(2) 通过 LATE-PCR 进行 DNA 扩增；(3) 试纸条加样；(4) 与捕获探针杂交；(5) 红点可视化检测。



图5  不同基因型的特异性杂交信号示例检测条。5 份样本均与预期分型结果匹配，符合率达 100%。

原文链接：Spotting of Multiplex Lateral Flow Assays for Blood Group Genotyping with the BIOSPOT® DX Platform | Knowledge Center


应用案例4 细胞培养
在高密度细胞板中在特定距离高密度点印细胞，实验高通量细胞共培养，观察不同时间点细胞与细胞之间的相互作用（图6）



图6 特定距离点印的细胞随着时间增殖并相互联系

 
代表客户


 


发表文献

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2.Eijkelboom NM, Gawronska K, Vollenbroek JM, Kraaijveld GJC, Boom RM, Wilms PFC, Schutyser MAI. Single droplet drying with stepwise changing temperature-time trajectories: Influence on heat sensitive constituents. Food Res Int. 2024 Apr;182:114194. doi: 10.1016/j.foodres.2024.114194. Epub 2024 Mar 3. PMID: 38519165.

3.Göckler T, Albreiki F, Li D, Grimm A, Mecklenburg F, Urueña JM, Schepers U, Srivastava S. Block Polyelectrolyte Additives That Modulate the Viscoelasticity and Enhance the Printability of Gelatin Inks at Physiological Temperatures. ACS Appl Polym Mater. 2024 Feb 21;6(5):2427-2441. doi: 10.1021/acsapm.3c01085. PMID: 38481474; PMCID: PMC10928658.

4.Fritschen A, Lindner N, Scholpp S, Richthof P, Dietz J, Linke P, Guttenberg Z, Blaeser A. High-Scale 3D-Bioprinting Platform for the Automated Production of Vascularized Organs-on-a-Chip. Adv Healthc Mater. 2024 Jul;13(17):e2304028. doi: 10.1002/adhm.202304028. Epub 2024 Apr 3. PMID: 38511587; PMCID: PMC11469029.

5.Zieger V, Woehr E, Zimmermann S, Frejek D, Koltay P, Zengerle R, Kartmann S. Automated Nanodroplet Dispensing for Large-Scale Spheroid Generation via Hanging Drop and Parallelized Lossless Spheroid Harvesting. Micromachines (Basel). 2024 Jan 31;15(2):231. doi: 10.3390/mi15020231. PMID: 38398960; PMCID: PMC10893090.

6.Sturm F, Zieger V, Koltay P, Frejek D, Kartmann S. Particle Detection in Free-Falling Nanoliter Droplets. Micromachines (Basel). 2024 May 31;15(6):735. doi: 10.3390/mi15060735. PMID: 38930704; PMCID: PMC11205310.

7.Zieger V, Frejek D, Zimmermann S, Miotto GAA, Koltay P, Zengerle R, Kartmann S. Towards Automation in 3D Cell Culture: Selective and Gentle High-Throughput Handling of Spheroids and Organoids via Novel Pick-Flow-Drop Principle. Adv Healthc Mater. 2024 Apr;13(9):e2303350. doi: 10.1002/adhm.202303350. Epub 2024 Feb 11. PMID: 38265410; PMCID: PMC11468932.

8.Vieira A, Jokinen V, Lepikko S, Ras RHA, Zhou Q. Through-Drop Imaging of Liquid-Solid Interfaces: From Contact Angle Variations Along the Droplet Perimeter to Mapping of Contact Angles Across a Surface. Langmuir. 2024 Apr 30;40(17):9059-9067. doi: 10.1021/acs.langmuir.4c00414. Epub 2024 Apr 15. PMID: 38621291; PMCID: PMC11072716.

9.Lai YK, Kao YT, Hess JF, Calabrese S, von Stetten F, Paust N. Interfacing centrifugal microfluidics with linear-oriented 8-tube strips and multichannel pipettes for increased throughput of digital assays. Lab Chip. 2023 May 30;23(11):2623-2632. doi: 10.1039/d3lc00339f. PMID: 37158238.

10.Lee J, Beukema M, Zaplatynska OA, O'Mahony C, Hinrichs WLJ, Huckriede ALW, Bouwstra JA, van der Maaden K. Efficient fabrication of thermo-stable dissolving microneedle arrays for intradermal delivery of influenza whole inactivated virus vaccine. Biomater Sci. 2023 Oct 10;11(20):6790-6800. doi: 10.1039/d3bm00377a. PMID: 37622228.