RTCA eSight 实时无标记细胞分析仪
基本信息
产品名称：
RTCA eSight 实时无标记细胞分析仪
英文名称：
xCELLigence RTCA eSight
国产/进口：
进口
产地/品牌：
Agilent (ACEA)
型号：
xCELLigence RTCA eS
参考报价：
销售商：
总点击数：
1707
更新日期：
2025-05-29
产品类别：
性能参数
特性
工作原理
第 1 步
接种细胞，然后将细胞板或组织培养容器放在培养箱中的 eSight 上。使用 eSight 软件设置数据和图像采集时间表。
第 2 步
添加治疗剂（如免疫效应细胞、抗体、抗病毒药物）
第 3 步
使用专有的 xCELLigence 生物传感技术自动采集数据，并实时叠加图像。
应用
Immune Cell Killing Assays
Typical immune cell killing assays are labor-intensive and require users to collect multiple timepoints using a combination of techniques. In contrast, the label-free xCELLigence RTCA instruments provide a complete view of immune cell killing and closely mimics activity in vivo. Obtain highly sensitive, reproducible, and direct measurements of immune cell killing potency in real time. This simple and high throughput workflow also provides greater insight into cellular mechanism of action, serial killing, and exhaustion.
Viral Cytopathic Effect Assays
Advance your anti-viral therapy or vaccine development by accurately measuring viral cytopathic effects (CPE) in real time with Agilent xCELLigence RTCA instruments, without the use of labor-intensive plaque assays. Monitor viral CPE automatically, from minutes to days, and greatly reduce workload and manual handling of samples. Measure cell proliferation kinetics, identify the optimal time point for viral infection with different cell seeding densities, and assess virus-mediated cytopathogenicity in one workflow.
Cytotoxicity Assays
Determine cytotoxic effects of drugs or compounds by monitoring cell proliferation, cell size or morphology, and cell-substrate attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload.
Apoptosis and Proliferation Assays
Learn about apoptosis and proliferation assays that are simple, quantitative, and high throughput to provide multiple perspectives into cell health and viability. Measure real-time cell health directly and capture early and late apoptotic events such as modulation of cell adhesion, cell membrane integrity, and cell detachment. Track proliferation of host cells to detect readout for drug toxicity or presence of a virus. Detect and quantify apoptosis in living cells by label-free approach, or using apoptotic or nuclear labeling reagents. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging).
Phagocytosis
Dynamically analyze key mechanisms by which the immune system removes pathogens and cell debris. Track the entire process in real-time by imaging, screen for pathway modulators, and discover therapeutic interventions. Label and visualize macrophages via nuclear labeling reagents.
- 将无标记 xCELLigence 实时细胞分析技术与具有红绿蓝三色荧光的成像技术相结合，增强对细胞分析及其结论的信心。
- 简单的工作流程能够适应不同的实验方案，可同时检测原代细胞或标准细胞系培养中的细胞活力、粘附强度、形态变化、增殖和死亡，提供生理相关的数据。
- 灵活的活细胞成像，支持明场、三个荧光通道、多种孔板形式，以及用户自定义实验流程。
- RTCA 能够提供细胞数量、增殖速率、细胞大小/形状和细胞-基质粘附强度的连续读数结果。
工作原理
第 1 步
接种细胞，然后将细胞板或组织培养容器放在培养箱中的 eSight 上。使用 eSight 软件设置数据和图像采集时间表。
第 2 步
添加治疗剂（如免疫效应细胞、抗体、抗病毒药物）
第 3 步
使用专有的 xCELLigence 生物传感技术自动采集数据，并实时叠加图像。
应用
Immune Cell Killing Assays
Typical immune cell killing assays are labor-intensive and require users to collect multiple timepoints using a combination of techniques. In contrast, the label-free xCELLigence RTCA instruments provide a complete view of immune cell killing and closely mimics activity in vivo. Obtain highly sensitive, reproducible, and direct measurements of immune cell killing potency in real time. This simple and high throughput workflow also provides greater insight into cellular mechanism of action, serial killing, and exhaustion.
Viral Cytopathic Effect Assays
Advance your anti-viral therapy or vaccine development by accurately measuring viral cytopathic effects (CPE) in real time with Agilent xCELLigence RTCA instruments, without the use of labor-intensive plaque assays. Monitor viral CPE automatically, from minutes to days, and greatly reduce workload and manual handling of samples. Measure cell proliferation kinetics, identify the optimal time point for viral infection with different cell seeding densities, and assess virus-mediated cytopathogenicity in one workflow.
Cytotoxicity Assays
Determine cytotoxic effects of drugs or compounds by monitoring cell proliferation, cell size or morphology, and cell-substrate attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload.
Apoptosis and Proliferation Assays
Learn about apoptosis and proliferation assays that are simple, quantitative, and high throughput to provide multiple perspectives into cell health and viability. Measure real-time cell health directly and capture early and late apoptotic events such as modulation of cell adhesion, cell membrane integrity, and cell detachment. Track proliferation of host cells to detect readout for drug toxicity or presence of a virus. Detect and quantify apoptosis in living cells by label-free approach, or using apoptotic or nuclear labeling reagents. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging).
Phagocytosis
Dynamically analyze key mechanisms by which the immune system removes pathogens and cell debris. Track the entire process in real-time by imaging, screen for pathway modulators, and discover therapeutic interventions. Label and visualize macrophages via nuclear labeling reagents.
公司简介
江苏睿捷生物成立于2011年，是省内生命科学领域专业的仪器、试剂、耗材提供商。公司专注于免疫学、细胞生物学、分子生物学等方向，为900多家终端单位和4000多客户提供创新技术与产品解决方案，与客户保持在实验前的调研准备和实验中的技术操作支持等全方位沟通。目前公司代理品牌有17个，其中仪器品牌有Agilent(Biotek 和ACEA），STEMCELL,JIMBIO,CURIOX
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