PureCol®EZGel即用型胶原蛋白溶液
PureCol®EZGel是一种即用型胶原蛋白溶液,只需在培养箱中加热至37°C即可形成牢固的凝胶。该产品由浓度为0.5%(〜5 mg / ml)的纯化的I型胶原蛋白,DMEM / F-12培养基以及L-谷氨酰胺和二肽(L-丙氨酸-L-谷氨酰胺)的混合物组成用于细胞培养的长效L-谷氨酰胺来源。
PureCol®EZGel旨在通过预先配制培养基并将产品调节至中性pH值来提高凝胶稠度。该产品避免了由于试剂添加,pH值调整和处理条件等因素引起的凝胶制备中的不一致。
PureCol®EZGel是提供牢固凝胶的理想选择,也可用于制备用于培养细胞的薄层。 PureCol®EZ Gel胶原蛋白以易于使用的包装形式提供,以供使用和存储。该产品是无菌的,可作为即用型溶液提供。
参数,测试和方法PureCol®EZ Gel I型胶原蛋白#5074
灭菌方法过滤
提取方法酶-端胶原
表格解决方案
包装尺寸35毫升
储存温度2-10°C
自收到之日起至少六个月的保质期
胶原蛋白浓度-双缩脲〜5 mg / mL
胶原蛋白纯度-银染色> 99%
pH值6.9-7.4
动凝胶测试(分钟)<40
凝胶形成管测试(分钟)<40
原纤维形成(吸光度单位)> 0.5
电泳图谱-考马斯亮蓝特征
无菌-USP改良无增长
内毒素-LAL <1.0 EU / mL
渗透压(mOsmo H2O / kg)300-360
细胞附着分析合格
来源牛皮
水凝胶杨氏模量E(Pa)特性
中等补品DMEM / F-12
L-谷氨酰胺和二肽(L-丙氨酸-L-谷氨酰胺)的L-谷氨酰胺来源混合物
Product Description
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
Parameter, Testing, and Method | PureCol® EZ Gel Type I Collagen #5074 |
Sterilization Method | Filtration |
Extraction Method | Enzyme - atelocollagen |
Form | Solution |
Package Size | 35 mL |
Storage Temperature | 2-10°C |
Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | ~5 mg/mL |
Collagen Purity - Silver Staining | >99% |
pH | 6.9-7.4 |
Kinetic Gel Test (Minutes) | <40 |
Gel Formation Tube Test (Minutes) | <40 |
Fibrillogenesis (Absorbance Units) | >0.5 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
Sterility - USP modified | No growth |
Endotoxin - LAL | <1.0 EU/mL |
Osmolality (mOsmo H2O/kg) | 300-360 |
Cell Attachment Assay | Pass |
Source | Bovine Hide |
Hydrogel Young's Modulus E (Pa) | Characteristic |
Medium Supplement | DMEM/F-12 |
L-Glutamine Source | Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |
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