通过调节气压(10 至250 psi), 系统适用于:

破坏性极小地打开细胞

彻底破碎细胞

对于许多细胞来说，仅通过1或2次操作就可使破碎率达到90%。

不产热，它反而使样品降温。

这个有效的过程只花极少的时间，因该装置能在30毫升/分的流量下破碎细胞。

工作原理

在这液滴的形成过程中, 悬浮在液体中的被雾化的大分子或细胞被强制分配，从液体形成二级液滴。

这产生了液面和所形成的二级液滴间中形成的微毛细管通道中的短暂的层流。

微毛细管通道中的短暂的层流能产出足够的剪切力来破碎细胞和大的刚性分子。


所产生的剪切力由所用的气压、气体的类型和液体的黏度。


这使得在雾化中精确调节所用的强度成为可能。


所以该仪器可用于细胞器的分离时破坏性极小地打开细胞之需，或细胞提取时彻底破碎细胞之需。

什么是BioNeb系统？
 
细胞破碎是指用于从细胞内获得感兴趣的生物分子的几种技术之一。最古老的方法可以追溯到20世纪40年代，当时引进了法国压机，这是一种新型的实验室仪器，使用液压活塞在样品缸内产生高压。这些压力迫使细胞通过狭窄的缝隙，破坏细胞壁和质膜，以支持细胞核、蛋白质和其他微生物的分离。
 
这项技术在当时是非同寻常的，但与21世纪的实验室仪器相比有严重的局限性。在广泛的生物化学和生命科学应用中，破碎活细胞以分离特定的微观分析物仍然是一个关键过程。要大规模地进行这项工作并达到新的精确度和可靠性水平，需要尖端的实验室仪器按照全新的碎裂原理进行操作。BioNeb细胞破碎系统旨在满足这些现代要求，以达到细胞雾化控制的新水平。
 
 
概述雾化的原理
 
法国媒体使用压力的物理感应来分裂细胞结构，而BioNeb实验室仪器则根据雾化原理工作。分析雾化器使用10–250 psi的气体压力的严格调节，将液体介质减少到非常细的雾状。悬浮在液体培养基中的细胞被强制分配到次级液滴中，次级液滴产生具有层流的微毛细管通道，层流表现出足够的剪切力来破坏细胞结构。
 
剪切力取决于三个主要因素:气体压力；气体成分；液体中等粘度。可以通过控制这些参数来微调产生的剪切力。例如，增加的介质粘度通常导致在较低压力操作下较高百分比的细胞破裂。
 
BioNeb实验室仪器随后能够雾化剪切显著不同的值。细胞只需三次就能被轻轻打开或完全破坏。也有可能在单程中实现多种细胞类型多达90%的破坏。这是一个高效的过程，流速为30毫升/分钟，并产生负热量。

What is the BioNeb System?
 
Cell disruption refers to one of several techniques used to obtain biological molecules of interest from within a cell. The oldest method is dated to the 1940s and the introduction of the French press, a novel laboratory instrument that induced high pressure within a sample cylinder using a hydraulic piston. These pressures forced cells through a narrow aperture, disrupting cell walls and plasma membranes to support isolation of nuclei, proteins, and other microorganisms.
This technique was extraordinary for its time but is severely limited compared to 21st Century laboratory instruments. Breaking apart living cells to isolate specific microscopic analytes remains a key process in a broad range of biochemical and life science applications. Performing this at scale and to new levels of both accuracy and reliability required cutting-edge laboratory instruments operating on entirely new fragmentation principles. The BioNeb® Cell Disruption System was engineered to meet these modern requirements to reach new levels of control for cell nebulization.
 
Outlining the Principles of Nebulization
Whereas the French press used physical induction of pressure to break apart cell structures, BioNeb® laboratory instruments operate on the principle of nebulization. An analytical nebulizer uses tight regulation of gas pressures from 10 – 250 psi to reduce a liquid medium to a very fine mist. Cells suspended in the liquid medium are forcefully distributed into the secondary droplet which creates a microcapillary channel with a laminar flow exhibiting sufficient shear force to break apart a cell structure.
Shearing forces are dependent on three primary factors: gas pressures; gas compositions; liquid medium viscosity. It is possible to finely tune the shearing forces generated by manipulating these parameters. For example, increased medium viscosity typically results in a higher percentage of cell breaking at lower pressure operation.
BioNeb® laboratory instruments are subsequently capable of nebulization shearing of significantly varied values. Cells can be gently opened or totally disrupted in just three passes. It is also possible to achieve as much as 90% disruption of numerous cell types in a single pass. This is a highly efficient process with flow rates of 30 ml/minute and negative heat generation.