此试剂盒提供用于 DNA-PAINT 成像的谷氨酸转运蛋白 1（VGLUT1）的即用型结合剂，VGLUT1 定位于兴奋性谷氨酸能突触的突触小泡膜上。

此外，还包含用于样本制备和成像的成像链和缓冲液。此试剂盒包含识别 VGLUT1 的单域抗体（sdAB）结合剂，且与 FAST DNA-PAINT 序列 F2 相偶联。每个 sdAB 均与一条单独的 DNA-PAINT 靠泊链相偶联。
未经修饰和与荧光团偶联的单域抗体（FluoTag®）可从 NanoTag 获得。

产品包含：

SINGLE DOMAIN ANTIBODY
· sdAB anti-VGLUT1 (clone: Nb9) + FAST docking site F2 (To be measured with
Imager F2 or F2-LB)
· Concentration: 5 µM Protein, 5 µM DNA (1 DNA strand per protein)
· Volume: 100 µL
· Storage buffer: PBS, 50 % glycerol + 0.05 % NaN3
· Storage: -20 °C
· Recommended dilution: 1:100-1:500 (for optimal results the dilution needs to be optimized
depending on the target accessibility and expression level)

IMAGERS
· Imager F2 or F2-LB Cy3B or ATTO 655
· Concentration: 1 µM in TE buffer (10 mM Tris, 1 mM EDTA, pH 8)
· Volume: 300 µL
· Storage: -20 °C (1 µM imager solutions are stable for multiple freeze-and-thaw cycles)
Optional: Prepare 50 µL aliquots and store at -20 °C. Working aliquots can be stored at 4 °C for
short-term or -20 °C for long term
Note: Further dilutions should be prepared fresh before use. Low imager concentrations are not
stable in plastic tubes.

BUFFERS
· Antibody incubation buffer, 30 mL, store at 2-8 °C
Note: For longer-term storage we recommend to store aliquots at -20 °C.
· Washing buffer (10×), 20 mL, store at room temperature (to be diluted 1:10 in water before use)
· Imaging buffer, 50 mL, store at room temperature