Aflatoxin (AFLA) is a mycotoxin which is produced by several Aspergillus species. Aflatoxin exposure produces an acute hepatic necrosis, resulting later in cirrhosis, and/or carcinoma of the liver. Acute hepatic failure is made manifest by hemorrhage, edema, alteration in digestion, and absorption and/or metabolism of nutrients and mental changes and/or coma. In line with various regulatory laws, it is required to control the AFLA content in food and feed.

Intended Use

Immunoaffinity columns are intended for use in a wide range of commodities including vegetable, fats, soy, vinegar etc. For this process, a representative sample of the commodity to be analysed is extracted with a mixture of solvent. The extract is filtered and then diluted with a buffer. The diluted extract can be run directly over the Immunoaffinity columns, in which the aflatoxins are bound and separated from the other substances of the extract. After a short rinsing phase isolated aflatoxins can be eluted using methanol. This eluate is suited for determining the concentration of aflatoxins directly with the HPLC.

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