内参抗体 Tubulin beta/TUBB
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Species Cross-reactivity H,M
Key Application ELISA,WB,ICC
Host Mouse
Antibody type Monoclonal antibody
Purified method Affinity purified
Isotype IgG1
Molecular Weight 50kDa
Sensitivity This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.
Immunogen Purified recombinant human Tubulin beta protein fragments expressed in E.coli.
UniProt Number P07437
GeneBank ID NM_178014
Subcellular location Cytoplasm
Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA
Altername beta1-tubulin,beta 5-tubulin,beta-4 tubulin,beta Ib tubulin class I beta-tubulin,tubulin beta-1 chain,tubulin beta-5 chain tubulin beta polypeptide,tubulin, beta polypeptide
Gene_symbol M40; TUBB1; TUBB5; OK/SW-cl.56
UniPort summary Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Entrez summary Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.
Application Images :

Western blot detection of TUBB in MCF7,Hela,K562,A431,3T3,Jurkat&Ramos cell lysates and using TUBB antibody (1:5000 diluted). Predicted band size: 50KDa Observed band size: 50KDa

Immunocytochemistry stain of Hela using TUBB antibody (1:300).
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